Descrição
Complement component 3 (C3), a pivotal molecule in the complement system, is an essential immune mediator in various diseases, including psoriasis. C3 deficiency promoted imiquimod-induced skin cell apoptosis and supported greater proportions of IFN-γ T cells in the inflamed tissues
Specifications
|
Synonyms |
Complement C3;HSE-MSF;C3;CPAMD1;AHUS5;ARMD9;ASP;C3a;C3b; |
|
Uniprot No. |
P01024 |
|
Source |
Recombinant Human Complement component 3 Protein is expressed from HEK293 cells with His tag at the C-terminal. It contains Ser23-Asn1663. |
|
Molecular Weight |
The protein has a predicted MW of 188.52 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 67-68 kDa, 110-115 kDa and 140-160 kDa based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
<1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Dissolve lyophilized protein with sterile water to ensure the concentration is greater than 100 ug/mL. |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable glove for your safety.
2.This product is for research use only.
Product Data
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Investigação
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