Descrição
Glypican 1, also known as GPC1, is a cell surface heparan sulfate proteoglycan that plays a crucial role in cell growth and development. It is a member of the glypican family, which are glycosylphosphatidylinositol-anchored proteins found on the cell surface.
Specifications
|
Synonyms |
Glypican 1, GPC1 |
|
Uniprot No. |
P35052-1 |
|
Source |
Recombinant Human Glypican 1/GPC1 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Asp24-Ser530. |
|
Molecular Weight |
The protein has a predicted MW of 57.17 kDa. And it migrates as 60-72 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
|
Purity |
> 90% as determined by SDS-PAGE. |
|
Biological Activity |
Human FGF-2 immobilized on CM5 chip can bind human GPC1, His tag, with an affinity constant of 5.83 × 10-9 M as determined in a SPR assay (Biacore 8K). |
|
Endotoxin |
< 1 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS. |
|
Reconstitution |
Centrifuge tubes before opening. Dissolve lyophilized protein with PBS. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Note
1.Please operate with lab coats and disposable gloves, for your safety.
2.This product is for research use only.
Product Data
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Investigação
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