Source

Human MMP-8 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity. It contains Phe21-Gly467.[Accession | P22894]

Molecular Weight

The protein has a predicted MW of 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE and HPLC

Activity

ELISA Data: Immobilized Human MMP-8, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 1.93μg/ml determined by ELISA.  

SPR Data: Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 μM as determined in SPR assay.

Enzyme Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 300 pmoles/min/μg

Formulation

Lyophilized from 0.22μm filtered solution in 50mM Tris, 10mM CaCl2, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.