Descrição
Specifications
|
Synonyms |
KLK5; Klnc;Kallikrein c; KLK-L2 |
|
Source |
Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val23-Ser293.[Accession | Q9Y337] |
|
Molecular Weight |
The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 42-48 kDa based on SDS-PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Activity |
ELISA Data: Immobilized Human Kallikrein 5, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. Bioactivity Data: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >200 pmol/min/µg. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 5.5). |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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Investigação
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