Descrição
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Product Alias |
MHC, RMF, LMP2, LMP-2, Macropain chain 7, Proteasome chain 7, PSMB9, RING12 |
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Species |
Human,Mouse |
|
Expression sequence |
Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119(B2M) and CLGGLLTMV peptide |
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Accession |
A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P61769(B2M)&CLGGLLTMV |
|
Label |
C-His-Avi |
|
Expression system |
HEK293 |
|
Molecular weight |
The protein has a predicted MW of 50.3 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
preparation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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Appearance |
Lyophilized Powder |
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Biological Activity |
【ELISA Data】Immobilized Chimeric HLA-A*02:01(m汐3)&B2M&LMP2 (CLGGLLTMV) Monomer, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. |
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Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing. |
Product Features
High purity, high activity, low endotoxin, high batch consistency.
Storage
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
Pagamento e segurança
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Investigação
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Perguntas frequentes
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