Figure 1. Hieff™ Magnetic Blood DNA Kit extracts more blood DNA than kits from other suppliers.
Descrição
The HieffTM Magnetic Blood DNA Kit provides fast and easy methods for purification of total DNA from blood samples. This product is based on the purification method of high binding magnetic particles. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The genomic DNA is suitable for various experiments, such as enzyme digestion, PCR,qPCR, etc.
Features
High yield and purity: OD260/280 ratio between 1.8–2.0.
Broad sample compatibility: Works with fresh blood, frozen blood, blood cells, and more.
Compatible with instruments: Suitable for high-throughput extraction on various automatic nucleic acid extractors.
Safe and non-toxic: Free of harmful substances like phenol and chloroform.
Components
|
Part |
Components No. |
Name |
18504ES50（50 rxn） |
18504ES70（200 rxn） |
|
Part I |
18504-A |
Proteinase K Solution（20 mg/mL） |
1 mL |
1 mL×4 |
|
Part II |
18504-B |
Magnetic beads Suspension |
1 mL |
1 mL×4 |
|
18504-C |
Lysis buffer |
15 mL |
60 mL |
|
|
18504-D |
Rinse buffer* |
52 mL |
70 mL×3 |
|
|
18504-E |
Wash buffer* |
16 mL |
22 mL×3 |
|
|
18504-F |
Elution buffer |
5 mL |
20 mL |
Shipping and Storage
Store Part I at 4°C and Part II at room temperature. The product is valid for one year.
Application
Blood gDNA extraction from blood, plasma, serum, or lymph samples.
Figures
Extraction workflow
Whole Blood RNA Extraction
|
kit |
18504 |
Supplier M* |
||||
|
Sample |
Nucleic Acids (ng/μL) |
260/280 |
260/230 |
Nucleic Acids (ng/μL) |
260/280 |
260/230 |
|
Sample 1 |
68.4 |
1.858 |
1.778 |
62.4 |
1.691 |
1.706 |
|
Sample 2 |
78.2 |
1.708 |
1.997 |
76.4 |
1.702 |
1.832 |
|
Sample 3 |
116 |
1.798 |
1.806 |
104 |
1.687 |
1.615 |
|
Sample 4 |
112 |
1.896 |
1.888 |
92.4 |
1.680 |
1.791 |
|
Sample 5 |
124.8 |
1.737 |
1.703 |
116.4 |
1.708 |
1.510 |
|
Sample 6 |
97.2 |
1.658 |
1.877 |
88.2 |
1.707 |
1.621 |
|
Mean Value |
99.43 |
1.78 |
1.84 |
89.97 |
1.70 |
1.68 |
300 μL of fresh blood in EDTA storage tubes was extracted simultaneously using the kits of 18504 and supplier M*, with a 96-channel nucleic acid extractor and 70 μL elution.
The results showed that the extraction rate of blood gDNA by 18504 was higher than that of supplier M*.
Supports extraction from swab samples
|
kit |
18504 |
Supplier K* |
||||||
|
Swab sample |
Concentration (ng/μL) |
260/280 |
260/230 |
Ct Mean |
Concentration (ng/μL) |
260/280 |
260/230 |
Ct Mean
|
|
Sample 1
|
3.015 |
1.901 |
1.227 |
9.901 |
4.907 |
0.998 |
0.011 |
10.778 |
|
3.102 |
1.807 |
1.157 |
9.344 |
14.454 |
0.516 |
0.028 |
8.641 |
|
|
3.542 |
1.724 |
1.187 |
9.547 |
5.652 |
0.688 |
0.016 |
7.514 |
|
|
Sample 2
|
5.333 |
1.982 |
1.794 |
8.539 |
6.041 |
1.571 |
0.009 |
7.788 |
|
7.466 |
1.803 |
1.702 |
7.823 |
6.765 |
1.022 |
0.021 |
9.414 |
|
|
7.139 |
1.975 |
1.721 |
6.777 |
7.203 |
0.645 |
0.024 |
10.893 |
|
|
Mean Value |
4.932 |
1.871 |
1.462 |
8.663 |
7.50 2 |
0.913 |
0.022 |
9.171 |
Figure 2. Performance of Hieff™ Magnetic Blood DNA Kit from swab sample
Test reagent: 18504ES and supplier K* ; elution volume: 70 μL.
Test scheme: AP-96 fully automated nucleic acid extractor; nanodrop detects DNA concentration and purity. 10 μL of each eluate was analyzed by qPCR using the HEK293 Residual DNA Detection Kit (Cat#41302).
Test results: 18504 showed brighter bands, higher purity, and lower average Ct values compared to the competitor
Documents:
Safety Data Sheet
Manuals
18504_Manual_Ver.EN20250606.pdf
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