The RT-LAMP Lyo-Ready Fluorescent Assay Kit (Bst Plus) is a kit capable of performing isothermal amplification using DNA and RNA as templates. This kit employs the next-generation aptamer-encapsulated Bst Plus DNA Polymerase and heat-resistant Hifair™ III Reverse Transcriptase, along with an MRI, combined with a finely optimized buffer system, to achieve efficient, rapid, and highly specific detection reactions. It also supports room-temperature reaction system preparation. The buffers and enzyme components in the kit do not contain glycerol and can be lyophilized using the provided lyophilization protectant.

Features

Broad Compatibility: Compatible with over 20 animal and human pathogen targets, with GC content ranging from 30% to 65%. Delivers comparable amplification times to leading products.

High Specificity: Validated across diverse DNA and RNA targets. Demonstrates superior specificity with no false positives within 60 minutes and delayed amplification in NTCs compared to competitors.

Excellent Sensitivity: Capable of detecting African swine fever virus at 15 copies/reaction and Influenza B virus at 10 copies/reaction.

Robust Stability: Maintains performance after 20 freeze-thaw cycles and 7 days at 37°C. Supports convenient room temperature reaction setup.

Components

Application

Isothermal Amplification;Rapid detection of pathogenic bacteria.

Figures

1. Workflow

Figure 1. RT-LAMP Workflow.

2. Consistent performance between lyophilized and liquid formats.

Figure 2. Comparable Detection Performance of Liquid and Lyophilized Formats.

Both liquid and lyophilized versions of the RT-LAMP assay exhibit consistent sensitivity and specificity in detecting DNA and RNA targets, with similar amplification curves and reaction times.