The FuniCutTM series of restriction endonucleases are rapidly acting, genetically engineered enzymes capable of precisely cutting DNA within 5 to 15 minutes. They are ideal for the rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. The FuniCutTM series shares a universal digestion buffer, simplifying the reaction setup, and offers excellent enzyme activity redundancy, making it easy to handle substrate excess or challenging templates.
Specifications
|
Enzyme |
Notl |
|
Recognition Site |
5'-GC↓GGCCGC-3' 3'-CGCCGG↑CG-5' |
|
Recommended Reaction Conditions |
1× FuniCutTM Buffer; incubate at 37°C. |
|
Enzyme Activity |
20 U/μL |
|
Inactivation Conditions |
Incubate at 80°C for 20 minutes. |
Components
|
Components No. |
Name |
15021ES50 |
|
15021-A |
FuniCutTM NotI |
50 μL |
|
15021-B |
10×FuniCutTM Buffer |
1 mL |
|
15021-C |
10×FuniCutTM Color Buffer* |
1 mL |
[Note]:*10xFuniCutTM Color Buffer includes red and yellow tracking dyes, allowing direct use of the product for gel electrophoresis. The red dye migrates similarly to a 2500 bp double-stranded DNA fragment in 1% agarose gel; the yellow dye migrates similarly to a 10 bp double-stranded DNA fragment in 1% agarose gel.
Shipping and Storage
This product should be stored at -25℃~-15℃ for up to 2 years.
Documents:
Safety Data Sheet
Manuals
15021_Manual_Ver.EN20250728.pdf
Storage
FAQ
Citations
Documents
Applications
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