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The FuniCutTM series of restriction endonucleases are rapidly acting, genetically engineered enzymes capable of precisely cutting DNA within 5 to 15 minutes. They are ideal for the rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. The FuniCutTM series shares a universal digestion buffer, simplifying the reaction setup, and offers excellent enzyme activity redundancy, making it easy to handle substrate excess or challenging templates.
Specifications
|
Enzyme |
Ncol |
|
Recognition Site |
5'-C↓CATGG-3' 3'-GGTAC↑C-5' |
|
Recommended Reaction Conditions |
1xFuniCutTM Buffer; optimal at 37℃ incubation |
|
Enzyme Activity |
20 U/μL |
|
Inactivation Conditions |
Incubate at 80℃ for 20 minutes |
|
Isoschizomers |
Bsp191 |
Components
|
Components No. |
Name |
15018ES30 |
|
15018-A |
FuniCutTM Ncol |
30 μL |
|
15018-B |
10 x FuniCutTM Buffer |
1 mL |
|
15018-C |
10 x FuniCutTM Color Buffer* |
1 mL |
[Note]: 10×FuniCutTM Color Buffer includes red and yellow tracking dyes, allowing direct loading onto agarose gels for electrophoresis. The red dye migrates similarly to a 2500 bp double-stranded DNA fragment in 1% agarose gel, while the yellow dye migrates similarly to a 10 bp double-stranded DNA fragment.
Shipping and Storage
This product should be stored at -25℃~-15℃ for up to 2 years.
Documents:
Safety Data Sheet
Manuals
15018_Manual_Ver.EN20250728.pdf
Storage
FAQ
Citations
Documents
Applications
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