Descrição
T7 Endonuclease I is encoded by gene 3 of bacteriophage T7. It recognizes and cleaves mismatched DNA, cruciform DNA structures, Holliday junctions (four-way DNA junctions), heteroduplex DNA, and also cleaves nicked double-stranded DNA at a slower rate. Cleavage occurs at the first, second, or third phosphodiester bond on the 5′ side of the mismatched base.
Features
High Cleavage Activity: 20 U of T7 Endonuclease I efficiently cuts 200 ng of mismatched dsDNA.
High Protein Purity: ≥95% (as determined by SDS-PAGE).
Specifications
|
Cat NO. |
14548ES72 / 14548ES86 |
|
Size |
250 U / 2500 U |
|
Source |
Recombinant expression in Escherichia coli of gene 3 from bacteriophage T7 |
|
Concentration |
10 U/μL |
|
Purity |
≥95% |
Components
|
Components No. |
Name |
14548ES72 |
14548ES86 |
|
14548-A |
T7 Endonuclease I (10 U/μL) |
25 μL |
250 μL |
|
14548-B |
10× T7 Endonuclease I Reaction Buffer |
1 mL |
1 mL |
Storage
This product should be stored at -25~-15℃ for 2 years.
Application
Detection of mutations following genome editing (e.g., CRISPR/Cas9 or TALEN-induced indels)
Resolution of four-way DNA junctions or branched DNA structures
Detection or cleavage of heteroduplex DNA and nicked DNA
Random fragmentation of linear DNA for shotgun cloning
Figures
1. High Cleavage Activity
Figure 1. Verification of T7 Endonuclease I cleavage performance (Input: 200 ng DNA)
Yeasen T7 Endonuclease I and imported Supplier A* T7 Endonuclease I were incubated with mismatched dsDNA substrates and analyzed by agarose gel electrophoresis. The results show that 20 U of Yeasen T7 Endonuclease I efficiently cleaves 200 ng of mismatched dsDNA, with a cleavage performance comparable to that of Supplier A*.
Documents:
Safety Data Sheet
Manuals
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Investigação
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