Descrição
Ribonuclease A (RNase A) is a non-specific endoribonuclease derived from bovine pancreas, engineered using protein engineering technology and produced via yeast expression and purification. It is free of bacterial endotoxins commonly associated with prokaryotic expression systems.
Functionally, RNase A is an endoribonuclease that hydrolyzes the phosphodiester bond between the 5′-ribose of a nucleotide and the 3′-ribose phosphate of an adjacent pyrimidine nucleotide (cytidine or uridine). This reaction generates a 2′,3′-cyclic phosphate intermediate, which is subsequently hydrolyzed to the corresponding 3′-nucleotide phosphate. Thus, RNase A specifically cleaves single-stranded RNA after C and U residues. RNase A is highly stable and exhibits strong activity against single-stranded RNA.
This product is widely used to remove RNA contamination from DNA or protein samples.
It is free of endonucleases, exonucleases, and proteases.
Features
Residue-Free: No residual DNA, RNA, DNase, or protease contamination
Animal-Origin Free: RNase A is produced by recombinant expression, with no animal-derived components
Precise and Efficient RNA Digestion: Specifically cleaves single-stranded RNA; widely used to remove RNA from DNA or protein preparations
Eukaryotic Expression System: Expressed and purified from yeast, free of bacterial endotoxins associated with prokaryotic expression
High Stability: RNase A is highly stable under a wide range of conditions
Application
Removal of RNA from DNA preparations
Removal of RNA from protein samples
Sample preparation for PCR, cloning, and sequencing
General molecular biology workflows requiring RNA digestion
Specifications
|
CAS No. |
9001-99-4 |
|
EC Number |
3.1.27.5 |
|
Source |
Recombinant yeast |
|
Molecular Weight |
14.3 kDa |
|
Dilution Buffer |
10 mM Tris-HCl (pH 8.0), 20 mM MgCl₂ |
|
Storage Buffer |
10 mM Tris-HCl (pH 8.0), 20 mM MgCl₂, 50% glycerol |
|
Appearance |
Light yellow, clear solution |
|
Stock concentration |
10 mg/mL |
|
Electrophoretic Purity |
≥90% (by SDS-PAGE) |
|
Enzyme Activity |
≥350 KU/mL |
|
DNA/RNA Residue |
None |
|
DNase Contamination |
None |
|
Protease Contamination |
<0.01 U/mg |
|
Optimal pH |
7.6 (functional range: pH 6–10) |
|
Optimal Temperature |
60°C (functional range: 15–70°C) |
Components
|
Name |
10418ES01 |
10418ES05 |
10418ES10 |
|
Recombinant RNase A (Liquid，DNase-Free ) |
1 mL |
5 mL |
10 mL |
Storage
This product should be stored at -25~-15℃ for one year.
Documents:
Safety Data Sheet
Manuals
Pagamento e segurança
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Investigação
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Perguntas frequentes
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