Lyticase is a specialized enzyme preparation for hydrolyzing yeast cell walls, widely used in downstream applications such as genome research, yeast artificial chromosome (YAC) extraction, yeast transformation, protoplast preparation, yeast cell disruption, industrial fermentation, and yeast inactivation.

This product is supplied in liquid form at a concentration of 10 U/μL, with a typical working concentration of 0.15 U/μL. The optimal concentration should be adjusted according to your specific application, yeast strain, and cell density. For genomic DNA or plasmid DNA extraction, please follow the recommended concentration in the corresponding kit protocol.

Features

Broad-Spectrum: Lyses a wide range of fungal cells for versatile applications.

Stable & Reliable: Consistent enzyme activity with easy storage and transport.

High-Efficiency: Rapid nucleic acid purification and high-quality protoplast generation.

Components

Storage

This Product should be stored at -25°C ~ -15°C. Valid for 2 years.

Application

• Yeast cell wall digestion
• Protoplast preparation from fungi
• Yeast transformation and genetic manipulation
• Extraction of genomic DNA or plasmid DNA from yeast
• Preparation of high-quality fungal RNA
• Industrial fermentation and yeast inactivation
• Fungal biotechnology research

Documents:

Safety Data Sheet

10403_MSDS_HB250909_EN.PDF

Manuals

10403_Manual_Ver.EN20250909.pdf

Citations & References:

[1] Lu H, Zhu J, Zhang T, et al. A rapid multiplex nucleic acid detection system of airborne fungi by an integrated DNA release device and microfluidic chip. Talanta. 2022;246:123467. doi:10.1016/j.talanta.2022.123467(IF:6.057)
[2] Liao X, Zhao J, Liang S, et al. Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastoris. Biotechnol Biofuels. 2019;12:38. Published 2019 Feb 21. doi:10.1186/s13068-019-1377-z(IF:5.452)