PNGase F (750U/μL) _20415ES

SKU: 20415ES01

Size: 15 KU
Price:
Sale price$155.00

Shipping calculated at checkout

Stock:
In stock
Bulk Quote Inquiry

Description

The PNGase F  is a recombinant enzyme expressed in yeast, which can cleave high mannoses, heterozygotes, and complex oligosaccharide proteins linked by asparagine. The cleavage site of PNGase F is the amide bond between N-acetylglucosamine (GlcNAc) and asparagic acid residue on the inner side of the glycoprotein, and the aspartyl on the enzymatic hydrolysis protein is converted to aspartic acid. This product has a His tag and is often used for complete deglycosylation of antibodies and their related proteins.

Component

No.

Name

20415ES01

20415ES02

20415-A

PNGase F

15 KU

75 KU

20415-B1

Buffer 1(10×)

150 μL

750 μL

20415-B2

Buffer 2(10×)

300 μL

1500 μL

20415-B3

10% NP-40

300 μL

1500 μL

 

产品性质

 

Name

PNGase F

Source

Expressed in Yeast

Molecular weight

36 kDa

Specific activity

750000 U/mL

Buffer

20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA,50% Glycerol

Unit Definition

One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µL.

 

Shipping and Storage

The products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.


Inquiry

You may also like