PNGase F (100U/μL) _ 20407ES

SKU: 20407ES01

Size: 15000 U
Price:
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Description

PNGase F is an amidase enzyme that is Cloned from Peace Station Eliza and mainly secreted by Neisseria meningitidis and other Gram-negative bacteria. Yeasen PNGase F is recombinantly expressed in yeast (specific activity: 100,000 U/mL), which can cleave high mannose, hybrid, and complex oligosaccharide glycoproteins linked by asparagine. The cleavage site of PNGase F is the amide bond between the inner N-acetylglucosamine (GlcNAc) and asparagine residue of glycoproteins, while converting the asparagine on the protein after enzymatic hydrolysis to aspartic acid. This product is tagged with His and is commonly used for complete deglycosylation of antibodies and related proteins.

Feature

High purity—No protease, glycosidase contamination, purity ≥95%.

High Activity 100,000 U/mL.

Good compatibilityCan be used denaturing or no-denaturing conditions.

Application

Glycan Sequencing

Proteomics

Glycoprotein Analysis

.Recombinant Glycoprotein Expression

Specification

English synonym

PNGase F; N-Glycosidase F; N-Glycosidase F

Source

Yeast recombinant expression

Molecular Weight

36 kDa

Specific Activity

100,000 U/mL

Storage buffer

20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA, 50% Glycerol

Unit Definition

1 unit of enzyme activity refers to the amount of enzyme required to remove more than 95% of the carbohydrates from 10 μg denatured RNase B in a 10 μL reaction system at 37°C within 1 hour.

Components

Components No.

Name

Component Composition

20407ES01

20407ES02

20407-A

 PNGase F

 PNGase F

15000 U

75000 U

20407-B1

Buffer 1 (10×)

5% SDS; 400 mM DTT

150 μL

750 μL

20407-B2

Buffer 2 (10×)

200 mM Tris, pH 7.5

300 μL

1500 μL

20407-B3

10% NP-40

10% NP-40 in MilliQ-H2O

300 μL

1500 μL

Storage

The product can be stored at -25 to -15℃ for one year.

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