Endo H _ 20414ES

SKU: 20414ES92

Size: 10000 U
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Description

Endo H is a recombinant glycosidase that can cleave the core structure of high-mannose and some hybrid oligosaccharides in N-glycoproteins, removing the N-linked high-mannose from glycoproteins. Endo H is cloned from the corrugated actinomycete (Streptomyces plicatus) and recombinantly expressed in yeast. This product is tagged with His and is commonly used for complete deglycosylation of antibodies and related proteins.

Feature

High purity—No protease, glycosidase contamination, purity ≥95%.

High Activity 1,000,000 U/mL.

Good compatibilityCan be used denaturing or no-denaturing conditions.

Application

Glycan Sequencing

Proteomics

Glycoprotein Analysis

Recombinant Glycoprotein Expression

Specification

English synonym

Endo H

Source

Yeast recombinant expression

Molecular Weight

60 kDa

Specific Activity

1,000,000 U/mL

Storage buffer

20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA

Unit Definition

One unit of endoglycosidase H is defined as the amount of enzyme required to hydrolyze >95% of the oligomannose from 10 μg of denatured RNase B in 1 hour at 37°C in a 10 μL reaction volume.

Components

Components No.

Name

20414ES92

20414ES97

20414-A

 Endo H

10000 U

50000 U

20414-B1

Buffer 1 (10×)

100 μL

500 μL

20414-B2

Buffer 2 (10×)

200 μL

1000 μL

Storage

The product can be stored at -25 to -15℃ for one year.

 

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