Endo S _ 20413ES
Endo S _ 20413ES
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Endo S _ 20413ESEndo S _ 20413ES
Endo S _ 20413ES
Endo S, is a highly specific glycosidase derived from Streptococcus pyogenes. It can cut the N-linked glycans between the core structure of the shell di-saccharides of wild-type IgG heavy chains. This product has a purity of over 95%, high activity, good stability, no glycosidase contamination, and no proteolytic activity. For easy subsequent operations, it is equipped with a histidine (His) tag, which makes it easy to remove from the reaction. Properties English synonym Endo S Source E.coli recombinant expression Purity Detected by SDS-PAGE, purity > 95% Storage buffer PBS pH 7.5...

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Endo S _ 20413ES

YeasenSKU: 20413ES80
Size: 1000 U
Price:
$70.00
Stock:
In stock

Endo S, is a highly specific glycosidase derived from Streptococcus pyogenes. It can cut the N-linked glycans between the core structure of the shell di-saccharides of wild-type IgG heavy chains. This product has a purity of over 95%, high activity, good stability, no glycosidase contamination, and no proteolytic activity. For easy subsequent operations, it is equipped with a histidine (His) tag, which makes it easy to remove from the reaction.

Properties

English synonym

Endo S

Source

E.coli recombinant expression

Purity

Detected by SDS-PAGE, purity > 95%

Storage buffer

PBS pH 7.5

Specific activity

8,000 U/mg

Unit Definition

The definition of a unit is that under a total reaction volume of 10 μL, at 37 °C for 1 hour, 95% of the N-linked glycans in 5 μg of natural mouse IgG can be removed.

Components

Components No.

Name

20413ES80

20413ES90

20413-A

Endo S

1000 U

5×1000 U

20413-B

10×Buffer

1 mL

5×1 mL

Instructions

Reaction systerm

Components

Volume

IgG

V

Endo S

1

10×Buffer

1

H2O

to 10

Note: After incubation at 37 °C for 1 hour, electrophoresis detection, load 2-3 μg of samples. The control group is the original protein.

Experimental Example

Electrophoresis of IgG under Endo S enzyme conditions

Lane -: Control, without Endo S enzyme; Lane +: With Endo S enzyme.

Notes

1. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety！

2. For research use only!

Storage

The product can be stored at -15~-25℃ for one year.

FAQ

Citations

Documents

Applications

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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.

Certain applications may require additional third-party intellectual property rights.

Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.