Descrizione
c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene .The protein possesses tyrosine kinase activity.
Specifications
|
Cat.No. |
96264ES25 / 96264ES60 / 96264ES76 |
|
Size |
25 μg / 100 μg / 500 μg |
Components
|
Name |
96264ES25 |
96264ES60 |
96264ES76 |
|
Recombinant Cynomolgus HGF R/c-MET Protein, His Tag, Ultra Low Endotoxin |
25 μg |
100 μg |
500 μg |
Performance parameters
|
Synonyms |
MET;oncogene MET;HGF R;HGF/SF receptor;AUTS9;cMET;Met (c-Met);RCCP2;SF receptor |
|
Species |
Cynomolgus |
|
Expression Sequence |
Glu25-Asn930 |
|
Accession |
A0A2K5UM36 |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 69.9 kDa (β chain). Due to glycosylation, the protein migrates to 45-52 kDa (α chain) and 75-82 kDa (β chain) based on Bis-Tris PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and SEC-HPLC |
|
Endotoxin |
Less than 0.01 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological activity |
ELISA Data 1:Immobilized Cynomolgus HGF R, His Tag at 5 μg/ml (100μl/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA . |
|
Reconstitution Method |
Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. |
Storage
Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves,for your safety.
Documents
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FAQ
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