In vitro mRNA synthesis produces mRNA for vaccines and gene therapy. A linearized DNA template with a promoter (e.g., T7 promoter: 5'-TAATACGACTCACTATAG-3' ) is transcribed by T7 RNA polymerase using nucleotides, buffer, and magnesium ions. A 5' cap is added via a capping enzyme or cap analog, and a poly-A tail is included using the template or poly-A polymerase for stability. DNase I removes residual DNA, and the mRNA is purified. Key enzymes—BspQ1, T7 RNA polymerase, Pyrophosphatase, Inorganic, Murine RNase inhibitor, capping enzyme, poly-A polymerase, and DNase I—ensure functional mRNA.
Salva $180.00
Yeasen
Cleascrip ™ T7 RNA polimerasi (dsRNA basso, 250 U/μl) _ 10628es
Prezzo di venditaDa $0.00
Prezzo regolare$180.00
Salva $198.00
Yeasen
Cleascrip ™ T7 RNA polimerasi (grado GMP, dsRNA basso, 250 U/μL) _ 10629es
Prezzo di venditaDa $0.00
Prezzo regolare$198.00
Salva $60.00
Yeasen
Kit di sintesi dell'RNA T7 ad alto rendimento -10623es
Prezzo di venditaDa $165.00
Prezzo regolare$225.00
Salva $40.00
Salva $28.00
Yeasen
Funicut ™ BSPQI GMP -grade (10 U/μL) -10664es
Prezzo di venditaDa $77.00
Prezzo regolare$105.00
Yeasen
MRNA VACCINIA CAPPAGGIO IN BAPPA GMP di livello GMP (10 U/μl) _ 10614es
Prezzo di venditaDa $365.00
Yeasen
MRNA Cap 2'-O-metiltransferasi GMP di livello (50 U/μl) _ 10612es
Prezzo di venditaDa $165.00
