Hieff™ Plant RNA Kit _ 19291ES
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Hieff™ Plant RNA Kit _ 19291ES
Hieff™ Plant RNA Kit _ 19291ES
The Hieff™ Plant RNA Kit is suitable for extracting RNA from polysaccharide polyphenol plants such as Arabidopsis thaliana, rice, maize, wheat, tomato, tobacco, cotton and Holly. The extraction and purification of total RNA from plant samples (100-200 mg fresh or cryopreserved samples) can be completed within 40 minutes without the use of toxic organic substances such as phenol and chloroform. The silicon matrix material used in the centrifugal adsorption column is a unique new material of our company, which can maximize the recovery of high-purity RNA with our unique lysis solution formula....

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Hieff™ Plant RNA Kit _ 19291ES

YeasenSku: 19291ES08
Size: 5 T
Prezzo:
$25.00
Azione:
In magazzino

The Hieff™ Plant RNA Kit is suitable for extracting RNA from polysaccharide polyphenol plants such as Arabidopsis thaliana, rice, maize, wheat, tomato, tobacco, cotton and Holly. The extraction and purification of total RNA from plant samples (100-200 mg fresh or cryopreserved samples) can be completed within 40 minutes without the use of toxic organic substances such as phenol and chloroform. The silicon matrix material used in the centrifugal adsorption column is a unique new material of our company, which can maximize the recovery of high-purity RNA with our unique lysis solution formula. DNase I directly digests gDNA on the column, and the extracted total RNA has high purity, stable and reliable quality, and can be applied to various downstream application experiments, such as RT-PCR, RT-qPCR, in vitro transcription, molecular cloning etc.

Features

Safety: no need to use organic reagents such as phenol chloroform.

Fast: RNA extraction from samples can be completed within 40 min minutes.

Widely compatibility: It is suitable for RNA extraction from Arabidopsis thaliana, rice, corn, wheat, tomato, tobacco, cotton, holly and other simple polysaccharide and polyphenol plant samples.

Low genome residue: equipped with DNase I, which can effectively remove DNA pollution.

Applications

Plant RNA extraction;Total RNA extraction from plants;

Specifications

Cat.No.

19291ES08 /19291ES50

Size

5 T / 50 T

Components

Category

Components No.

Name

19291ES08

19291ES50

Part I

19291-A

DNase Buffer

250 μL

1.25 mL ×2

19291-B

DNase I (RNase-free)

25 μL

250 μL

Part II

19291-C

RNA Adsorption Column P4 (RNA Column P4)

5

50

19291-D

2 mL Collection Tube P4

5

50

19291-E

Lysate LB (LB Buffer P4)

5 mL

50 mL

19291-F

Deproteinized solution PL (PL Buffer P4)

4 mL

40 mL

19291-G

Wash Buffer W*

1.3 mL

13 mL

19291-H

RNase-free H2O

1 mL

5 mL

Figures

RNA was extracted from Rice, corn, Arabidopsis, tomato. The extracted RNA was then subjected to agarose gel electrophoresis, and the electrophoretogram is shown below.

Table 1. High quality of extracted products. 

Table 1. High quality of extracted products.

Storage

Part I should be stored at 2~8℃ for 2 years.

Part II should be stored at room temperature for 2 years.

FAQ

You can give it a try.
Cotton, coniferous trees, ginkgo; the flesh of bananas and grapes; the seeds of wheat and peanuts; fungi such as mushrooms, etc.
DNA filtration column: Specifically adsorbs DNA from the tissue lysate and filters out the solid substances in the lysate. RNA adsorption column: Specifically adsorbs RNA.
The samples were repeatedly subjected to freezing and thawing, or there was contamination with RNase during the extraction process.
If the starting amount of the sample is too high or the material is not fully lysed, it is recommended to use a smaller amount of sample and thoroughly grind or homogenize it.
The initial sample input volume was too high. The initial input volume should be reduced. Alternatively, in subsequent experiments, a reverse transcription kit with the removed genomic material can be selected for removal.

Citations

Documents

Manual
19291ES-Manual-Ver.EN20260722.pdf
MSDS
19291ES--MSDS-HB250409.pdf

Applications

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Indagine

FAQ

Il prodotto è solo per scopi di ricerca e non è destinato all'uso terapeutico o diagnostico su esseri umani o animali. Prodotti e contenuti sono protetti da brevetti, marchi e copyright di proprietà di Yeasen Biotechnology. I simboli dei marchi indicano il paese di origine, non necessariamente la registrazione in tutte le regioni.

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Yeasen è un sostenitore della scienza etica, convinto che la nostra ricerca debba affrontare questioni critiche garantendo al contempo sicurezza e standard etici.