Enterokinase (also known as enteropeptidase, EC 3.4.21.9) is a key enzyme that initiates protein digestion in the intestine. It precisely activates trypsinogen to trypsin, which then cascade-activates chymotrypsinogen and procarboxypeptidase. Its expression is specifically localized in differentiated intestinal cells and goblet cells, making it both a core regulator of nutritional digestion and a reliable marker of intestinal epithelial maturation.
The Enterokinase Activity Assay Kit is a rapid method for determining enterokinase activity in biological samples. This method detects enterokinase activity by cleaving a 7-amino-4-trifluoromethylcoumarin (AFC)-labeled peptide substrate containing an enterokinase recognition sequence. The intensity of the fluorescent product AFC (excitation wavelength Ex=380 nm, emission wavelength Em=500 nm) generated by the reaction is proportional to the enzyme activity in the sample.
Product Information
|
Component No. |
Component Name |
20393ES60 (100 T) |
|
20393-A |
200× Enterokinase Substrate |
24 μL |
|
20393-B |
1 mM AFC Standard |
100 μL |
|
20393-C |
Enterokinase Assay Buffer |
60 mL |
Properties
|
Cat# |
Component Name |
20393ES60 (100T) |
|
20393-A |
200x Enterokinase Substrate |
24 uL |
|
20393-B |
1 mM AFC Standard |
100 uL |
|
20393-C |
Enterokinase Assay Buffer |
60 mL |
Figures
Figure 1. AFC standard curve.
Figure 2. Sensitivity of the Yeasen Enterokinase (Cat#20395ES) detection kit. Data were collected at 25°C for 60 min according to the kit instructions.
Storage
FAQ
Citations
Documents
| Brochure |
|---|
| Industrial_Production_Solutions_for_Tirzepatide_and_Semaglutide.pdf |
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Pertanyaan
FAQ
Produk ini hanya untuk keperluan penelitian dan tidak ditujukan untuk penggunaan terapeutik atau diagnostik pada manusia atau hewan. Produk dan konten dilindungi oleh paten, merek dagang, dan hak cipta milik
Aplikasi tertentu mungkin memerlukan hak kekayaan intelektual pihak ketiga tambahan.