| PCR Master Mix | panjang | ekstensi | gc | pewarna | Hot Start | 3 ’end | Fidelity | Aplikasi |
| ≤10-15 kb | 1-10 dtk/kb | 30-70% | ROSE RED | √ | 3'-da | 1 × | Genotipe, PCR Koloni, PCR Cepat | |
| ≤5 kb | 30 detik/kb | 40-70% | biru | × | 3'-da | 1 × | PCR reguler, genotipe | |
| ≤5 kb | 30 detik/kb | 40-70% | tidak berwarna | × | 3'-da | 1 × | PCR reguler | |
| ≤4 kb | 30 detik/kb | 30-70% | biru | √ | 3'-da | 1 × | Genotipe tikus | |
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| pcr langsung | ||||||||
| ≤10-15 kb | 1-10 dtk/kb | 30-70% | ROSE RED | √ | 3'-da | 1 × | Colony Direct PCR | |
| ≤1 kb | 30 S/KB | 30-70% | biru | √ | 3'-da | 1 × | mouse langsung PCR | |
| ≤8 kb | 10 s/kb untuk ≤8 kb | 30-75% | tidak berwarna | √ | 3'-da | 1 × | PCR Direct Darah | |
| ≤1 kb | 60 S/KB | 40-65% | biru | √ | 3'-da | 1 × | Plant Direct PCR | |
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| jaringan mouse/ reagen lisis sel | |
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| PCR Fidelity Tinggi | ||||||||
| ≤16 kb | 5 dtk/kb | 20-80% | biru | √ | Blunt | 83 × TAQ | premix | |
| ≤13 kb | 30 dtk/kb | 30-60% | tidak berwarna | √ | Blunt | 83 × TAQ | enzim | |
| ≤13 kb | 30 detik/kb | 30-60% | biru | √ | Blunt | 83 × TAQ | premix | |
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| Kit Kloning | no.Fragmen | panjang fragmen | volume | premix | dsDNA | SSDNA |
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| 1-6 | 50 bp-25 kb | 20μL atau 6μL | ya | √ | × |
| Satu Langkah Kloning Kit | |
transkripsi terbalik dan campuran master qPCR real-time
| qpcr | deteksi | rox | Sensitivitas | Spesifisitas | GC tinggi | Komponen |
| berbasis pewarna | NO/LOW/ROX TINGGI | 9 | 9 | 9 | campuran qPCR biru | |
| Berbasis Pewarna | No ROX | 9 | 9 | 8 | campuran qPCR biru + Diluen templat kuning | |
| berbasis pewarna | ROX rendah | 9 | 9 | 8 | campuran qpcr biru + pengenceran templat kuning | |
| berbasis pewarna | rox tinggi | 9 | 9 | 8 | campuran qpcr biru + pengenceran templat kuning | |
| berbasis pewarna | no/rendah/rox tinggi | 9 | 9 | 9 | miRNA qPCR + H2O | |
| probe berbasis | NO/LOW/ROX TINGGI | 10 | 9 | 8 | Mix Master | |
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| 50 × Referensi Referensi Pewarna |
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| rt-qpcr | penentuan posisi produk | multiplex qpcr | sensitivitas | spesifisitas | tinggi gc | platform kompatibel |
| berbasis pewarna |
| 9 | 9 | 8 | no/rendah/rox tinggi | |
| berbasis probe | √ | 10 | 9 | 8 | NO/LOW/ROX TINGGI | |
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| transkripsi terbalik | suhu RT | GC tinggi atau template kompleks | waktu RT | penghilang gDNA | untuk qpcr | Sub-Aplikasi |
| 55℃ | tinggi | 15 mnt | √ | √ |
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| 37℃ | bagus | 50 mnt | × | √ | miRNA | |
| 50-55℃ | Tinggi | 5 menit | √ | √ |
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| 37℃ | bagus | 5 mnt | √ | √ |
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| 50-55℃ | tinggi | 5 mnt | √ | √ | pcr, ≤14 kb |
elektroforesis asam nukleat
Kategori | Cat. no | Nama Produk | aplikasi |
Agarose | Agarose | Elektroforesis Asam Nukleat Rutin | |
Agarose high eyeving (kelas PCR) | Cocok untuk memisahkan fragmen DNA 20 bp-800 bp, sebanding dengan gel poliakrilamida | ||
tablet agarosa (0.5 g/tablet) | nyaman untuk aplikasi agarose rutin | ||
Pewarna asam nukleat | Pewarnaan gel asam nukleat tahunan (10.000 × air) | larut dalam air, dengan sifat spektral mirip dengan EB, excitable pada 300 nm lampu UV | |
penanda DNA | Goldband 1 KB DNA Ladder | 250-12000 bp (13 pita) | |
Goldband 50 bp DNA Ladder | 50-1000 bp (14 pita) | ||
Goldband 100bp DNA Ladder | 100-1500 bp (12 pita) | ||
goldband 100 bp plus ladder DNA | 100-3000 bp (14 pita) | ||
Goldband 200 bp DNA Ladder | 200-5000 bp (12 pita) | ||
Goldband 500 BP DNA Ladder | 500-5000 bp (8 pita) | ||
Goldband DL2000 DNA Marker | 100-2000 bp (6 pita) | ||
Goldband DL5000 DNA Marker | 100-5000 bp (9 pita) | ||
Goldband DL10,000 DNA Marker | 100-10000 bp (10 pita) | ||
Goldband Ladder DNA skala goldband | 100-12000 bp (20 band) | ||
Goldband DL15000 DNA Marker | 250-15000 bp (7 pita) |
