In vitro mRNA synthesis produces mRNA for vaccines and gene therapy. A linearized DNA template with a promoter (e.g., T7 promoter: 5'-TAATACGACTCACTATAG-3' ) is transcribed by T7 RNA polymerase using nucleotides, buffer, and magnesium ions. A 5' cap is added via a capping enzyme or cap analog, and a poly-A tail is included using the template or poly-A polymerase for stability. DNase I removes residual DNA, and the mRNA is purified. Key enzymes—BspQ1, T7 RNA polymerase, Pyrophosphatase, Inorganic, Murine RNase inhibitor, capping enzyme, poly-A polymerase, and DNase I—ensure functional mRNA.
Menyimpan $180.00
Yeasen
Cleascrip ™ T7 RNA polimerase (dsRNA rendah, 250 u/μl) _ 10628es
Harga penjualanDari $0.00
Harga reguler$180.00
Menyimpan $60.00
Menyimpan $198.00
Yeasen
Cleascrip ™ T7 RNA polimerase (GMP-grade, dsRNA rendah, 250 U/μl) _ 10629es
Harga penjualanDari $0.00
Harga reguler$198.00
Menyimpan $40.00
Menyimpan $28.00
Yeasen
FUNICUT ™ BSPQI GMP -Grade (10 U/μl) -10664es
Harga penjualanDari $77.00
Harga reguler$105.00
Menyimpan $15.00
Yeasen
T7 RNA polimerase GMP -grade (50 U/μl) -10624es
Harga penjualanDari $55.00
Harga reguler$70.00
Menyimpan $35.00
Yeasen
T7 RNA polimerase GMP -grade (250 U/μl) -10625es
Harga penjualanDari $105.00
Harga reguler$140.00
