Keterangan
This product is a phage T7 RNA polymerase derived from recombinant expression in E. coli. It is an optimized mutant of wild-type T7, formulated as a mixture of T7 RNA polymerase variants that significantly reduce dsRNA content. Using double-stranded DNA containing the T7 promoter sequence as a template and NTPs as substrates, it synthesizes RNA complementary to the strand downstream of the promoter. Double-stranded linear blunt-ended or 5'-overhanging DNA can serve as substrate templates for T7 RNA polymerase; therefore, linearized plasmids and PCR products can both be used as templates for in vitro RNA synthesis. Modified nucleotides can be added to the substrates during transcription to prepare biotin- or dye-labeled RNA.
Specifications
|
Catalog No. |
10632ES20 / 10632ES60 / 10632ES70 / 10632ES80 |
|
Size |
20 μL / 100 μL / 200 μL / 1 mL |
Component Information
|
Component |
10632ES20 |
10632ES60 |
10632ES70 |
10632ES80 |
|
T7 RNA Polymerase Mix (low dsRNA) |
20 μL |
100 μL |
200 μL |
1 mL |
Storage
Store at -25°C to -15°C. Valid for 1 year.
Documents:
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FAQ
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