The Hieff™ Multiplex PCR Master Mix for NGS is a ready-to-use 2× premix developed for multiplex PCR applications. It contains a hot-start, multiplex-optimized Taq DNA polymerase blend and a proprietary amplification buffer system.

The master mix enables rapid and convenient multiplex PCR and supports efficient amplification of targets with GC content ranging from 25% to 75%. It delivers high uniformity, specificity, and sensitivity across multiple amplicons.

This reagent is suitable for multiplex amplification of 30–300 amplicons of different sizes and can also be used for capture-based assays involving thousands or more amplicons. It is widely applicable in animal and plant breeding, forensic identification, genetic disease testing, food safety analysis, and related fields.

Features

Easy to operate: Ready-to-use 2× premixed solution

Broad Compatibility: Suitable for complex samples, GC-rich/poor systems(25-75%), and multiplex amplification.

Excellent performance: High uniformity, specificity, and sensitivity; supports 30–300 multiplex amplicons or enrichment of thousands of targets.

Components

Application

Targeted gene panels: Amplify disease-related genes (e.g., cancer, inherited disorders).

Direct blood PCR: No DNA extraction needed.

Forensic identification: Type STRs/SNPs for identity.

Agricultural breeding: Detect trait-associated markers (e.g., SNPs, indels).

Figures

1. High Amplification Efficiency