Hieff Trans Booster DNA RNA Transfection

Hieff Trans™ Booster DNA/RNA Transfection Reagent _ 40801ES

4.8 ( 8900 reviews)

Booster DNA/RNA Transfection Reagent is an advanced nucleic acid transfection reagent developed based on novel polymeric materials. Its unique molecular design significantly reduces cytotoxicity and features a smart anti-enzymatic degradation mechanism that protects nucleic acids from intracellular enzymatic degradation during delivery, thereby maximizing transfection efficiency. This reagent is suitable for various types of nucleic acids including DNA, siRNA, miRNA, mRNA, and ASO, and has been validated for efficient transfection in cell lines such as 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW267.4, HCT116, as well as primary cells, providing reliable tools for gene research.

Features

Low Cytotoxicity: The novel polymer replaces traditional liposomes to reduce cytotoxicity and improve cell viability.

Fast Release and Higher Efficiency: Fast release minimizes intracellular nucleic acid degradation, achieving >90% transfection efficiency (measured by flow cytometry).

Premium Options for Difficult-to-transfect cell lines: Sensitive and primary cells.

Proven Performance, Broad Cell Line Coverage: Achieves high efficiency in 200+ various cell lines including 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, and HCT116 and primary cells, with consistent and reproducible results.

One Reagent, All Nucleic Acids: Delivers DNA, siRNA, miRNA, mRNA, and ASO with high efficiency—even up to 15 kb fragments.

Components

Components No.	Name	40801ES01	40801ES04
40801-A	Booster DNA/RNA Transfection Reagent	100 μL	1.5 mL
40801-B	Transfection Enhancer	100 μL	1.5 mL

Shipping and Storage

This product should be stored at 2~8℃ for 12 months.

Application

Cell Transfection; DNA Transfection; mRNA/SiRNA Transfection;

Figures

1) High-Efficiency DNA Transfection

Figure 1. DNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior DNA transfection efficiency of Booster.

2) High-Efficiency mRNA Transfection

Figure 2. mRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior mRNA transfection efficiency of Booster.

3) High-Efficiency siRNA Transfection

Figure 3. siRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior siRNA transfection efficiency of Booster.

Customer Feedback

1) High-Efficiency Primary Dermal Fibroblasts Transfection

Figure 5. Plasmid EGFR DNA transfection in primary dermal fibroblasts using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.

2) High-Efficiency Porcine Alveolar Macrophages Transfection

Figure 6. DNA transfection in primary Porcine Alveolar Macrophages (PAMs) using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.

Documents:

Safety Data Sheet

40801_MSDS_HB250715_EN.PDF

Manuals

40801_Manual_Ver.EN20250807.pdf

Product Brochure

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View Details

Beyond Liposomes:
The Next Generation Booster DNA/RNA Transfection

High toxicity, low efficiency, and poor compatibility have long been the three major challenges in cell transfection. Traditional transfey. For primary or sensitive cells, they often require tedious optimization of experimental conditionction reagents, such as lipid-based reagents, offer good efficiency but exhibit high toxicits. PEI-based transfection reagents, while low in toxicity, suffer from poor compatibility; apart from 293 cells, their transfection efficiency in other cell types tends to be weak.
Based on in-depth biological and materials science research, Yeasen has newly developed a next-generation polymeric transfection reagent——Hieff Trans^TM Booster DNA&RNA Transfection Reagent.

Key Highlights

Low Cytotoxicity:

The novel polymer replaces traditional liposomes to reduce cytotoxicity and improve cell viability.

Fast Release and Higher Efficiency:

Fast release minimizes intracellular nucleic acid degradation, achieving >90% transfection efficiency (measured by flow cytometry).

Premium Options for Difficult-to-transfect cell lines:

Sensitive and primary cells.

Proven Performance, Broad Cell Line Coverage:

Achieves high efficiency in 200+ various cell lines including 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, and HCT116 and primary cells, with consistent and reproducible results.

One Reagent, All Nucleic Acids:

Delivers DNA, siRNA, miRNA, mRNA, and ASO with high efficiency—even up to 15 kb fragments.

Case Studies and Protocols

Click Cell Name to View Detailed Transfection Protocols

Cell Type	Abbreviation	Nucleic Acid Type
Primary mouse skin fibroblasts	MSFs	DNA
Primary human skin fibroblasts	HSFs	DNA
Primary mouse hepatocytes	PMHs	DNA
Primary mouse neural stem cells	mNSCs	mRNA
Primary human pulmonary artery endothelial cells	HPAECs	DNA
Primary bone marrow–derived macrophages	BMDMs	siRNA
Primary chicken embryo fibroblasts	CEFs	DNA
Primary porcine skeletal muscle satellite cells	PSCs(or PMSCs)	DNA
Primary glioblastoma cells	GBMs	siRNA
Primary porcine alveolar macrophages	PAMs	DNA
Human umbilical vein endothelial cells	HUVEC	DNA
Primary cardiac fibroblasts from neonatal mice	PMCF	siRNA

Cell Type	Abbreviation	Nucleic Acid Type
Human T-lymphoblastic leukemia cells	Jurkat	DNA
Human cervical cancer cells	Hela	DNA
Mouse colon cancer cells	MC38	DNA
Human gastric adenocarcinoma cells	AGS	DNA
Human hepatocellular carcinoma cells	HepG2	DNA
Human colon cancer cells	HCT-116	siRNA
Human gastric cancer cells	HGC-27	siRNA
Human bladder transitional cell carcinoma cells	T24	DNA
Human non-small cell lung carcinoma cells	A549	DNA
Mouse Lewis lung carcinoma cells	LLC	siRNA
Rat adrenal pheochromocytoma cells	PC-12	DNA
Human gastric carcinoma cells	SNU-719	siRNA
Human monocytic leukemia cells	THP-1	siRNA
Human osteosarcoma cells	U2OS	DNA
Human glioblastoma cells	U251	DNA
Human colorectal carcinoma cells	HCT-116	DNA
Human T-Lymphocyte leukemia cells	Jurkat	DNA
Human breast cancer cells	MCF-7	siRNA
Human pancreatic cancer cells	MIA PaCa2	DNA
Human lung squamous carcinoma cells	NCI-H520	siRNA
Human nasopharyngeal carcinoma cells	S18	DNA
Human monocytic leukemia cells	THP-1	DNA
Human glioblastoma cells	U251	siRNA

Cell Type	Abbreviation	Nucleic Acid Type
Mouse monocyte–macrophage cells	RAW264.7	DNA
Mouse monocyte–macrophage cells	RAW264.7	siRNA
Insect ovarian cells	Sf9	DNA
Silkworm (Bombyx mori) ovarian cells	BmN	DNA
Human cardiomyocyte cells	AC16	DNA
Mouse embryonic pre-osteoblast cells	MC3T3-E1	DNA
Mouse embryonic pre-osteoblast cells	MC3T3-E1	siRNA
Human embryonic kidney cells	HEK293	DNA
Human embryonic kidney cells	HEK293	mRNA
Mouse embryonic fibroblast cells	NIH-3T3	DNA
Bovine mammary epithelial cells	MACT	DNA
Human keratinocyte cells	HaCaT	DNA
Chicken embryonic fibroblast cells	DF-1	DNA
Mandarin fish (Siniperca chuatsi) cells	Mandarin fish (Siniperca chuatsi) cells	DNA + mRNA co-transfection
Human cardiomyocytes	AC16	siRNA
Mouse myoblasts	C2C12	DNA
Human umbilical vein endothelial hybrid cells	EAhy926	DNA
Rat cardiomyocytes	H9C2	DNA
Mouse monocyte–macrophages	RAW264.7	DNA
Mouse normal hepatocytes cells	Aml12	siRNA
Mouse bone marrow–derived dendritic cells	DC2.4	DNA
H9C2 rat cardiomyoblasts	H9C2	siRNA
HK-2 human renal proximal tubular epithelial cells	HK-2	siRNA
Neural progenitor cells	NPC	DNA
Mouse monocyte–macrophage cells	RAW264.7	siRNA

Unlock Creativity with Booster's Superior Performance

AI-powered Novel Transfection Reagent Development Platform

Leveraging an AI-powered and high-throughput screening platform for transfection reagent development, Yeasen Biotechnology has successfully launched a series of transfection reagents covering three major fields: basic scientific research, recombinant protein/antibody production, and viral vector manufacturing.

This platform provides researchers with high-quality foundational transfection reagents. It's high-performance products support large-scale antibody and protein production; and it also offers GMP-grade transfection reagents for viral packaging, meeting the demands of large-scale AAV and lentiviral (LV) vector production, thereby robustly supporting drug development and manufacturing applications.

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Unique Mechanism Enhances Transfection Efficiency at Every Step.

Hieff Trans^TMBooster is a next-generation polymeric transfection reagent. Its unique mechanism enhances transfection efficiency at every step, delivering higher success rates for your transfection experiments!

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1) High Loading Capacity → Dual mechanism: charge interaction + polymer chain entanglement; Higher loading capacity than traditional charge-interaction methods.

2) Efficient Cellular Uptake → Membrane translocation; Faster than conventional endocytosis pathways.

3) Non-Damaging Release → Rapid polymer degradation by intracellular enzymes/reducing GSH; Zero-damage release of nucleic acids.

4) Enhanced Safety → Rapid polymer degradation property; Lower toxicity than traditional lipid-based transfection reagents.

Cell Transfection FAQs

How do I mix multiple plasmids for co-transfection?

Keep total DNA within kit maximum. Each plasmid ≥10 % of total. Adjust molar ratio, reporter plasmid can be reduced 1:5 to 1:10 to avoid "squelching".

When should I assay knock-down after siRNA transfection?

mRNA: 24–48 h.
Protein: 48–72 h.
FAM-siRNA fluorescence visible as cytoplasmic puncta within 6 h.

What’s different about DNA, mRNA, and siRNA transfection?

DNA: Requires nuclear entry for transcription. Timing may be cell cycle-dependent.
mRNA: Only needs to reach the cytoplasm. Faster expression onset, no risk of genomic integration. expression starts 1–4 h, lasts 1–4 days. Ideal for hard-to-transfect cells, short-term expression, promoter-independent studies.
siRNA: Small duplex RNA that mediates sequence-specific mRNA degradation. Expression knockdown can appear within hours. Shorter incubation (4–24 hours), requires specific targeting sequences, used for gene silencing.

How should I improve transfection in primary cells?

Primary cells are often sensitive and have lower uptake efficiency.
Tips:
Use reagents validated for primary cells.
Optimize cell confluency (often 60–80%).
Minimize reagent toxicity (lower doses, shorter exposure).
educe serum concentration during transfection (or use serum - compatible reagents).
Minimize exposure time to the transfection complex (4–6 hours).
Consider electroporation for hard-to-transfect types.

What are the characteristics of common commercial transfection reagents?

Lipid-based:
Lipofectamine^TM, FuGENE^TM, jetPEI^TM. Strengths: High efficiency, broad applicability. Limitations: Can be costly

Polymer-based:
PEI, TurboFect^TM, JetPEI. Strengths: Cost-effective, scalable. Limitations: Higher cytotoxicity

Electroporation:
Lonza Nucleofector^TM, NeonTM. Strengths: Suitable for hard-to-transfect cells. Limitations: Requires specialized equipment

Calcium phosphate:
Classic method. Strengths: Low cost. Limitations: Variable reproducibility

Yeasen Booster is a next-generation polymeric transfection reagent, which has lower toxicity, higher efficiency.