विवरण
This product is a multiplex qPCR premix utilizing the company's next-generation antibody-based hot-start Taq enzyme.
Powered by Yeasen's ZymeEditor™ enzyme engineering platform, researchers performed comprehensive directed evolution of Taq DNA polymerase through structural analysis, multi-source sequence alignment, site-directed mutagenesis, random mutagenesis screening, stress testing, and enzyme fragment recombination. Using MTPS high-throughput screening technology, candidates were evaluated across thermal stability, affinity, polymerase activity, and exonuclease activity. The resulting Taq DNA polymerase exhibits enhanced inhibitor tolerance and fast processive synthesis. The molecular diagnostics raw material R&D platform further optimized the qPCR reaction system through extensive clinical sample testing and comparison with market alternatives, officially launching this qPCR premix specifically designed for African swine fever virus (ASFV) detection. Customized kit collaborations with ASFV primers and probes are also available.
Feature
- Compatibility: Optimized for ASFV nucleic acid detection, particularly compatible with Hongshi and Tianlong real-time PCR instruments.
- Sensitivity: Demonstrated higher detection rate and sensitivity than comparable market products across hundreds of clinical samples.
- Fast: Results available within 20 minutes.
- Stability: Premixed primers and probes remain stable after 7 days at 37°C and 20 freeze-thaw cycles.
- dUTP/UDG contamination prevention system: Incorporates a dUTP/UDG system that effectively prevents aerosol contamination.
Figure
Case Study 1: ASFV Clinical Sample Detection
|
Instrument |
Tianlong |
Reaction System |
25 μL total volume, including 5 μL of template |
|
Template |
Real clinical samples |
Template Concentration |
Ct values are shown below |
|
Primers and Probe |
African swine fever virus (ASFV) primers and probe |
Pretreatment |
Nucleic acids extracted on a large scale from pig farm samples using the magnetic bead method |
|
Amplification Program |
95°C for 10 sec; 40 cycles (95°C for 1 sec; 60°C for 5 sec) - 20 min |
||
Figure 1. Amplification curves of 45 ASFV clinical samples.
Table 1. Ct value statistics of 45 ASFV clinical samples.
Results: Forty-five clinical samples were tested in parallel using commercially available qPCR premixes for African swine fever virus (ASFV) detection. The results showed that Yeasen 16967 achieved a higher ASFV detection rate, stronger fluorescence signals, and better detection performance for low-concentration samples.
Case Study 2: Sensitivity Detection
|
Instrument |
Tianlong |
Reaction System |
25 μL total reaction volume, including 5 μL of template |
|
Template |
Real clinical samples |
Template Concentration |
Serially diluted to the limit of detection (LoD) |
|
Primers and Probe |
African swine fever virus (ASFV) primers and probe |
Pretreatment |
Nucleic acids extracted on a large scale from pig farm samples using the magnetic bead method |
|
Amplification Program |
95°C for 10 sec; 40 cycles (95°C for 1 sec; 60°C for 5 sec); total run time: approximately 20 min |
||
Table 2. Sensitivity comparison of ASFV detection using qPCR premixes from different manufacturers.
Results: Two serially diluted African swine fever virus (ASFV) nucleic acid samples at the limit of detection (LoD) were tested in parallel using comparable commercially available qPCR premixes for ASFV detection. The results showed that Yeasen 16967 demonstrated slightly better sensitivity and Ct values than comparable products on the market.
Case Study 3: Stability Test
|
Instrument |
Tianlong |
Reaction System |
25 μL total reaction volume, including 5 μL of template |
|
Template |
African swine fever virus (ASFV) positive control |
Template Concentration |
Positive control concentration and sensitivity-level concentration |
|
Primers and Probe |
African swine fever virus (ASFV) primers and probe |
Pretreatment |
Magnetic bead-based extraction |
|
Amplification Program |
95°C for 10 sec; 40 cycles (95°C for 1 sec; 60°C for 5 sec); total run time: approximately 20 min |
||
Figure 2. Stability test of 16967 with premixed ASFV primers and probes after 7 days at 37°C.
Results: The 16967 premix containing African swine fever virus (ASFV) primers and probe was incubated at 37°C for 7 days and compared with the reagent stored routinely at −20°C. Both reagents were used to amplify the ASFV positive control, nucleic acid at the sensitivity-level concentration, and NTC. The results showed no significant differences in amplification curve profiles, Ct values, or detection rates between the two storage conditions. In addition, no nonspecific amplification was observed after thermal acceleration, indicating that the fully premixed product has good stability.
Storage
Store at -25°C to -15°C, valid for 18 months.
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यह उत्पाद केवल शोध उद्देश्यों के लिए है और मनुष्यों या जानवरों में चिकित्सीय या नैदानिक उपयोग के लिए अभिप्रेत नहीं है। उत्पाद और सामग्री येसेन बायोटेक्नोलॉजी के स्वामित्व वाले पेटेंट, ट्रेडमार्क और कॉपीराइट द्वारा संरक्षित हैं। ट्रेडमार्क प्रतीक मूल देश को इंगित करते हैं, जरूरी नहीं कि सभी क्षेत्रों में पंजीकरण हो।
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