Description
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene .Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF).
Specifications
|
Cat.No. |
96273ES25 / 96273ES60 / 96273ES76 |
|
Size |
25 μg / 100 μg / 500 μg |
Components
|
Name |
96273ES25 |
96273ES60 |
96273ES76 |
|
Recombinant Cynomolgus MSLN/Mesothelin Protein, His Tag, Ultra Low Endotoxin |
25 μg |
100 μg |
500 μg |
Performance parameters
|
Synonyms |
MSLN;CAK1 antigen;CAK1;Mesothelin;MPF;MPFSMRP;SMR; |
|
Species |
Cynomolgus |
|
Expression Sequence |
Asp296-Ser598 |
|
Accession |
XP_005590873.3 |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 34.87 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and SEC-HPLC |
|
Endotoxin |
Less than 0.01 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Reconstitution Method |
Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. |
Storage
Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles.
ApplicationCase
ELISA Data 1: Immobilized Cynomolgus MSLN, His Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 0.12 μg/mL determined by ELISA.
ELISA Data 2: Immobilized Cynomolgus MSLN, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.8 ng/mL determined by ELISA.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves,for your safety.
Documents
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Enquête
FAQ
Le produit est destiné à des fins de recherche uniquement et n'est pas destiné à un usage thérapeutique ou diagnostique chez l'homme ou l'animal. Les produits et le contenu sont protégés par des brevets, des marques déposées et des droits d'auteur appartenant à Yeasen Biotechnology. Les symboles de marque indiquent le pays d'origine, pas nécessairement l'enregistrement dans toutes les régions.
Certaines applications peuvent nécessiter des droits de propriété intellectuelle tiers supplémentaires.
Yeasen se consacre à la science éthique, estimant que nos recherches doivent répondre à des questions cruciales tout en garantissant la sécurité et les normes éthiques.