Description
IL-33 is a 30 kDa pro-inflammatory protein. IL-33 is constitutively expressed in smooth muscle and airway epithelium. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following stimulation with IL-1 alpha or IL-1 beta. The C-terminal fragment of mature IL-33 binds to and triggers signaling through the mast cell IL-1 R4/ST2L, an orphan receptor long implicated in enhancing Th2 cell responses. Subsequently, IL-33 and ST2L associate with IL-1R AcP to form a ternary signaling complex. Stimulation of Th2-polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion. Administration of mature IL-33 in vivo promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues. Full-length and mature human IL-33 share 52-58% amino acid sequence identity with mouse and rat IL-33.
This product is provided as a lyophilized powder and features high purity, high activity, and low endotoxin.
Product Information
|
Product Information. |
Catalog Number |
Size |
|
Recombinant Human PDGF-AB Protein |
90369ES05 |
2 μg |
|
90369ES10 |
10 μg |
|
|
90369ES50 |
50 μg |
|
|
90369ES60 |
100 μg |
|
|
90369ES80 |
1 mg |
Performance Parameters
|
Synonyms |
IL33, IL-33, interleukin 33, Interleukin-1 family member 11, interleukin -33 |
|
Source |
E.coli |
|
Accession |
O95760 |
|
Molecular Weight |
Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing159 amino acids. |
|
AA Sequence |
Ser112-Thr270 |
|
Tag |
No Tag |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Purity |
> 97% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 10^7 IU/mg. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PBS, 150 mM NaCl, 1 mM EDTA, pH7.4. |
|
Appearance |
Lyophilized Powder |
Storage
Store at -25 ~ -15°C. Valid for 1 year upon receipt.
After reconstitution, store at 2 ~ 8°C for up to 30 days, or at -85 ~ -65°C for up to 3 months.
Reconstitution Method
Centrifuge before opening the vial to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, and can be further diluted according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer. Aliquot into single-use portions, freeze at -80°C, and avoid repeated freeze-thaw cycles.
Notes
1. For your safety and health, please wear a lab coat and disposable gloves during operation.
2. This product is for research use only.
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FAQ
Le produit est destiné à des fins de recherche uniquement et n'est pas destiné à un usage thérapeutique ou diagnostique chez l'homme ou l'animal. Les produits et le contenu sont protégés par des brevets, des marques déposées et des droits d'auteur appartenant à Yeasen Biotechnology. Les symboles de marque indiquent le pays d'origine, pas nécessairement l'enregistrement dans toutes les régions.
Certaines applications peuvent nécessiter des droits de propriété intellectuelle tiers supplémentaires.
Yeasen se consacre à la science éthique, estimant que nos recherches doivent répondre à des questions cruciales tout en garantissant la sécurité et les normes éthiques.