Description
HieffTM Endo-free Plasmid Mini Kit is designed for rapid extraction of 20–50 µg of high-purity plasmid DNA from 1–10 mL of E. coli LB culture. This kit features high efficiency, speed, and convenience. The spin column utilizes a proprietary silica-based matrix material developed by our company. Combined with our unique lysis buffer formulation, it enables maximum recovery of high-purity DNA. The extracted DNA has high purity and extremely low endotoxin residue (<0.1 EU/µg DNA), resulting in excellent performance in cell transfection. The DNA can be directly used in various molecular biology applications such as restriction digestion, transformation, PCR, in vitro transcription, and sequencing.
Feature
- Stable performance: Specially designed silica membranes ensure consistent DNA binding and reliable results.
- Fast and easy: Extract 20–50 µg high‑copy plasmid DNA from 1.5–5 mL E. coli LB culture in about 30 minutes.
- Pure DNA: Removes endotoxins to below 0.1 EU/µg DNA for excellent cell transfection.Specifications
Components
|
Category |
Components No. |
Name |
19021ES08 |
19021ES50 |
19021ES70 |
|
Part I |
19021-A |
RNase A (10 mg/mL) |
15 µL |
125 µL |
500 µL |
|
19021-B |
Endotoxin Removal Solution ER (ER Solution E2) |
500 µL |
5 mL |
20 mL |
|
|
Part II |
19021-C |
Buffer RS* (RS Buffer E2*) |
1.5 mL |
12.5 mL |
50 mL |
|
Part III |
19021-D |
Buffer AC (AC Buffer E2) |
500 µL |
5 mL |
20 mL |
|
19021-E |
DNA Binding Column E2 (HieffTM DNA Column E2) |
5 pcs |
50 pcs |
200 pcs |
|
|
19021-F |
2 mL Collection Tube (2 mL Collection Tube E2) |
5 pcs |
50 pcs |
200 pcs |
|
|
19021-G |
Lysis Buffer LB (LB Buffer E2) |
1.5 mL |
12.5 mL |
50 mL |
|
|
19021-H |
Binding Buffer BD (BD Buffer E2) |
1.5 mL |
12.5 mL |
50 mL |
|
|
19021-I |
Protein Lysis Buffer PL* (PL Buffer E2*) |
1.6 mL |
16 mL |
63 mL |
|
|
19021-J |
Wash Buffer W* (Wash Buffer*) |
1.3 mL |
13 mL |
50 mL |
|
|
19021-K |
Elution Buffer |
1 mL |
10 mL |
20 mL |
Shipping and Storage
Store Part I components at -25°C ~ -15°C; store Part II components at 2~8°C;
store Part III components at room temperature. The product is valid for 1 year.
Application
plasmid DNA Extraction; Endo-free Plasmid Extraction; Plasmid preparation for transfection.
Figures
1. Superior plasmid quality with ultra‑low endotoxin
Figure 1. Plasmid DNA extracted using Yeasen 19021, Supplier O*, and Supplier T* kits was transfected into HEK293 cells with pEGFP‑C1. Bright‑field and green‑fluorescence images were taken at 72 h post‑transfection, showing transfection efficiency in the order Yeasen 19021> Supplier T* > Supplier O*.
Documents:
Safety Data Sheet
Manuals
19021_Manual_Ver.EN20250806.pdf
Citations & References:
[1] Han P, Cao P, Yue J, et al. Knockdown of hnRNPA1 Promotes NSCLC Metastasis and EMT by Regulating Alternative Splicing of LAS1L exon 9. Front Oncol. 2022;12:837248. Published 2022 Jun 23. doi:10.3389/fonc.2022.837248(IF:5.738)
[2] Zhang J, Wang W, Feng N, Jiang X, Zhu S, Chen YQ. Ndufa6 regulates adipogenic differentiation via Scd1. Adipocyte. 2021;10(1):646-657. doi:10.1080/21623945.2021.2007590(IF:4.534)
[3] Zhu S, Zhang J, Wang W, Jiang X, Chen YQ. Blockage of NDUFB9-SCD1 pathway inhibits adipogenesis : Blockage of NDUFB9-SCD1 pathway inhibits adipogenesis. J Physiol Biochem. 2022;78(2):377-388. doi:10.1007/s13105-022-00876-7(IF:4.158)
Paiement et sécurité
Vos informations de paiement sont traitées en toute sécurité. Nous ne stockons pas les détails de la carte de crédit ni accès aux informations de votre carte de crédit.
Enquête
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FAQ
Le produit est destiné à des fins de recherche uniquement et n'est pas destiné à un usage thérapeutique ou diagnostique chez l'homme ou l'animal. Les produits et le contenu sont protégés par des brevets, des marques déposées et des droits d'auteur appartenant à Yeasen Biotechnology. Les symboles de marque indiquent le pays d'origine, pas nécessairement l'enregistrement dans toutes les régions.
Certaines applications peuvent nécessiter des droits de propriété intellectuelle tiers supplémentaires.
Yeasen se consacre à la science éthique, estimant que nos recherches doivent répondre à des questions cruciales tout en garantissant la sécurité et les normes éthiques.