The Hieff™ Plasmid Mini Kit adopts Hieff™ DNA Column P3 and a new solution system, and uses the conventional alkali cracking method to separate plasmid DNA. In the extraction process, toxic organic substances such as phenol and chloroform are not needed to be extracted, and time-consuming isopropanol or ethanol precipitation is not needed. The Hieff™ DNA Column P3 in the kit can specifically adsorb plasmid DNA, and does not adsorb protein, polysaccharide and other non-nucleic acid substances, so it is efficient, rapid and convenient. The extracted plasmid DNA has high purity and can be directly used in subsequent experiments such as DNA digestion, transformation and sequencing.
Feature
- Wide Sample Range: Suitable for routine small-scale plasmid extraction; processes 2–6 mL of bacterial culture per prep.
- High DNA Purity: Free from protein and RNA contamination; DNA is ready for downstream applications such as digestion, transformation, and sequencing.
- High DNA Yield: Columns can bind up to 60 μg of plasmid DNA.
Components
|
Components No. |
Name |
19001ES08 |
19001ES50 |
19001ES70 |
|
|
5 T |
50 T |
200 T |
|||
|
Part I |
19001-A |
RNase A (10 mg/mL) |
15 µL |
125 µL |
500 µL |
|
Part II |
19001-B |
RS Buffer P3* |
1.5 mL |
12.5 mL |
50 mL |
|
19001-C |
AC Buffer P3 |
500 µL |
5 mL |
20 mL |
|
|
19001-D |
Hieff™ DNA Column P3 |
5 |
50 |
200 |
|
|
19001-E |
2 mL Collection Tube P3 |
5 |
50 |
200 |
|
|
19001-F |
Lysis Buffer(LB Buffer P3) |
1.5 mL |
12.5 mL |
50 mL |
|
|
19001-G |
Binding Buffer(BD Buffer P3) |
1.8 mL |
17.5 mL |
70 mL |
|
|
19001-H |
Deproteinization Buffer(PL Buffer P3*) |
1.6 mL |
16 mL |
63 mL |
|
|
19001-I |
Wash Buffer* |
1.3 mL |
13 mL |
50 mL |
|
|
19001-J |
Elution Buffer |
1 mL |
10 mL |
20 mL |
|
Shipping and Storage
Part I components are transported in ice bags and stored at -25~-15℃;
Part II components are transported at room temperature and stored at room temperature.
The product is valid for 12 months.
Application
Plasmid Extraction
Figures
1. High Yields
Figure 1. Comparison of Plasmid DNA Yields from Equal Volumes of Bacterial Culture
Plasmid yield: 19001 > Supplier M > Supplier F.
Documents:
Safety Data Sheet
Manuals
Related Blog
Storage
FAQ
Citations
Documents
Applications
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FAQ
Le produit est destiné à des fins de recherche uniquement et n'est pas destiné à un usage thérapeutique ou diagnostique chez l'homme ou l'animal. Les produits et le contenu sont protégés par des brevets, des marques déposées et des droits d'auteur appartenant à Yeasen Biotechnology. Les symboles de marque indiquent le pays d'origine, pas nécessairement l'enregistrement dans toutes les régions.
Certaines applications peuvent nécessiter des droits de propriété intellectuelle tiers supplémentaires.
Yeasen se consacre à la science éthique, estimant que nos recherches doivent répondre à des questions cruciales tout en garantissant la sécurité et les normes éthiques.