Enterokinase (also known as enteropeptidase, EC 3.4.21.9) is a key enzyme that initiates protein digestion in the intestine. It precisely activates trypsinogen to trypsin, which then cascade-activates chymotrypsinogen and procarboxypeptidase. Its expression is specifically localized in differentiated intestinal cells and goblet cells, making it both a core regulator of nutritional digestion and a reliable marker of intestinal epithelial maturation.
The Enterokinase Activity Assay Kit is a rapid method for determining enterokinase activity in biological samples. This method detects enterokinase activity by cleaving a 7-amino-4-trifluoromethylcoumarin (AFC)-labeled peptide substrate containing an enterokinase recognition sequence. The intensity of the fluorescent product AFC (excitation wavelength Ex=380 nm, emission wavelength Em=500 nm) generated by the reaction is proportional to the enzyme activity in the sample.
Product Information
|
Component No. |
Component Name |
20393ES60 (100 T) |
|
20393-A |
200× Enterokinase Substrate |
24 μL |
|
20393-B |
1 mM AFC Standard |
100 μL |
|
20393-C |
Enterokinase Assay Buffer |
60 mL |
Properties
|
Cat# |
Component Name |
20393ES60 (100T) |
|
20393-A |
200x Enterokinase Substrate |
24 uL |
|
20393-B |
1 mM AFC Standard |
100 uL |
|
20393-C |
Enterokinase Assay Buffer |
60 mL |
Figures
Figure 1. AFC standard curve.
Figure 2. Sensitivity of the Yeasen Enterokinase (Cat#20395ES) detection kit. Data were collected at 25°C for 60 min according to the kit instructions.
Storage
FAQ
Citations
Documents
| Brochure |
|---|
| Industrial_Production_Solutions_for_Tirzepatide_and_Semaglutide.pdf |
También te puede gustar
Consulta
Preguntas frecuentes
El producto es solo para fines de investigación y no está destinado a uso terapéutico o diagnóstico en humanos o animales. Los productos y el contenido están protegidos por patentes, marcas comerciales y derechos de autor propiedad de Yeasen Biotechnology. Los símbolos de marca comercial indican el país de origen, no necesariamente el registro en todas las regiones.
Algunas aplicaciones pueden requerir derechos de propiedad intelectual adicionales de terceros.
Yeasen se dedica a la ciencia ética y cree que nuestra investigación debe abordar cuestiones críticas al tiempo que garantiza la seguridad y los estándares éticos.