Descripción
The Hieff™ Magnetic Circulating Cell-Free DNA Kit is suitable for extracting circulating DNA from 0.3-4 ml human plasma and serum. This product adopts unique magnetic beads and carefully optimized buffer system, which can maximize the purification and recovery of circulating DNA (typically 1-100 ng/ml circulating DNA in human plasma). The extracted nucleic acid is suitable for various downstream applications, such as NGS, qPCR, etc.
Feature
High Yield: Delivers high nucleic acid concentration with excellent recovery of short fragments.
Superior Quality: Produces high-quality libraries for downstream sequencing.
Broad Compatibility: Works with 0.3–4 mL plasma samples from both cfDNA-specific and standard EDTA tubes.
Components
|
Components No. |
Contents |
18386ES12 |
18386ES48 |
|
|
12 T |
48 T |
|||
|
Part I |
18386-A |
Proteinase K (20 mg/mL) |
1.25 mL×2 |
10 mL |
|
18386-B |
Magnetic Bead Suspension |
750 μL ×2 |
6 mL |
|
|
Part II |
18386-C |
Lysis Buffer |
2.5 mL |
10 mL |
|
18386-D |
Binding Buffer |
65 mL |
125 mL ×2 |
|
|
18386-E |
Wash Buffer A |
80 mL |
320 mL |
|
|
18386-F |
Wash Buffer B* |
22 mL |
88 mL |
|
|
18386-G |
Elution Buffer |
5 mL |
15 mL |
|
Storage
Both components can be transported at room temperature. For storage, Component I should be kept at 2–8°C, while Component II can be stored at room temperature. Both have a shelf life of 18 months.
Figure
Case Study 1: Superior cfDNA Recovery from Healthy Donor Plasma
Using a 50 µL elution volume, the 18382 kit demonstrated outstanding recovery efficiency from 2 mL of healthy donor plasma collected in cfDNA preservation tubes. Quantitative analysis confirmed that the kit delivers high nucleic acid yields, ensuring sufficient template material for sensitive downstream molecular applications.
|
Sample |
Competitor N (Conc. ng/µL) |
Supplier N (Total ng) |
18382 (Conc. ng/µL) |
18382 (Total ng) |
|
2 mL Healthy Plasma-1 |
0.296 |
14.80 |
0.437 |
21.85 |
|
2 mL Healthy Plasma-2 |
0.262 |
13.10 |
0.308 |
15.40 |
|
2 mL Healthy Plasma-3 |
0.378 |
18.90 |
0.410 |
20.50 |
|
2 mL Healthy Plasma-4 |
0.525 |
21.00 |
0.600 |
24.00 |
|
Average |
0.365 |
16.95 |
0.439 |
20.44 |
Case Study 2: High-Integrity cfDNA Extraction from EDTA Plasma
Using a 20 µL elution volume, the 18382 kit achieved excellent recovery efficiency from 2 mL of healthy donor EDTA plasma. Fragment analysis revealed distinct characteristic peaks at 170 bp and 340 bp, confirming the high integrity of the extracted cfDNA. Notably, no high-molecular-weight DNA interference was observed, ensuring high-purity nucleic acids ready for downstream sequencing or PCR assays.
Figure 1. cfDNA yield and fragment analysis from 2 mL EDTA plasma using the 18382 kit.
Documents:
Safety Data Sheet
Manuals
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Preguntas frecuentes
El producto es solo para fines de investigación y no está destinado a uso terapéutico o diagnóstico en humanos o animales. Los productos y el contenido están protegidos por patentes, marcas comerciales y derechos de autor propiedad de Yeasen Biotechnology. Los símbolos de marca comercial indican el país de origen, no necesariamente el registro en todas las regiones.
Algunas aplicaciones pueden requerir derechos de propiedad intelectual adicionales de terceros.
Yeasen se dedica a la ciencia ética y cree que nuestra investigación debe abordar cuestiones críticas al tiempo que garantiza la seguridad y los estándares éticos.