This product is a dedicated adapter set for Tn5 and Tn5-related transposases.
The series contains 8 i5 Index Primers (N5XX) and 12 i7 Index Primers (N7XX), which can be combined with each other to construct a total of 96 different dual-index libraries. The kit is available in two specifications, which can construct 24 (4 x 6) or 96 (8 x 12) dual-index libraries, respectively. The newly optimized adapters significantly improve sample amplification efficiency and library demultiplexing rate.
Product Information
|
Product Name |
Catalog No. |
Size |
|
Hieff NGS™ Tagment Index Kit for Illumina™ (24 Index) |
17265ES24 |
24×2 T |
|
Hieff NGS™ Tagment Index Kit for Illumina™ (96 Index) |
17265ES96 |
96×2 T |
|
Hieff NGS™ Tagment Index Kit for Illumina™ (384 Index) |
17265ES97 |
384×2 T |
Product Components
|
Product No. |
Name |
Size |
|
17265ES24 |
N501–N504 |
12 µL each |
|
N701–N706 |
8 µL each |
|
|
17265ES96 |
N501–N508 |
24 µL each |
|
N701–N712 |
16 µL each |
|
|
17265ES97 |
N501–N516 |
48 µL each |
|
N701–N724 |
32 µL each |
Feature
No amplification bias for transposase-based library construction.
Excellent uniformity.
Up to 96 combinations of indexes.
Applications
1. CUT&Tag library construction result figure
Figure 1: CUT&Tag library construction result. Using the dedicated Tn5 adapter, compatible with the Illumina sequencing platform. The library shows no bias.
2. ATAC-seq library construction result figure
Figure 2: ATAC-seq library construction result. Using the dedicated Tn5 adapter, compatible with the Illumina sequencing platform. The library shows no bias.
Storage
FAQ
Citations
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Applications
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