Descripción
This kit is an enhanced system based on the principle of topoisomerase-mediated rapid and efficient DNA fragment ligation. Compared with traditional T4 DNA ligase, it offers several significant advantages.
Features
Rapid Reaction: Ligation is completed in just 1–5 minutes.
High Efficiency: Nearly 100% positive cloning rate with no self-ligation; blue-white screening is unnecessary.
Simplified Workflow: From ligation to plating takes only 15–20 minutes. The protocol eliminates ice bath, heat shock, and 1-hour recovery steps.
Broad Compatibility: Efficiently ligates DNA fragments up to 5 kb.
Specifications
|
Product type |
Cloning Kit |
|
Principle |
TA Cloning: Topoisomerase |
|
Antibiotic-resistant Bacteria |
Ampicillin (AmpR) |
|
Methods of cloning |
TA |
Components
|
Components No. |
Name |
10907ES20 (20T) |
10907ES50 (50T) |
|
10907-A |
pESI-T vector (30 ng/μL) |
20 μL |
50 μL |
|
10907-B |
1 kb control insert (40 ng/μL) |
5 μL |
5 μL |
|
10907-C |
10× Enhancer |
20 μL |
50 μL |
Shipping and Storage
This product should be stored at -25~-15℃ for 12 months.
Application
TA Cloning; Gene Expression Vector Construction; Rapid Cloning of PCR Products; Sequence Verification.
Figures
1. Principle
Figure 1. Principle of TOPO Cloning
2. Workflow
Figure 2. Workflow of TOPO Cloning
3. Superior Cloning Efficiency
Figure 2. Cloning Efficiency of Kit 10907 Across Fragment Sizes and Comparison with Supplier A*
Left(A–C): Colony formation after TA cloning using Kit 10907 with inserts of 1.2 kb (A), 3 kb (B), and 5 kb (C), showing robust cloning efficiency across a broad size range.
(D–F): PCR verification of positive clones for each insert size, confirming successful insertion of 1.2 kb (D), 3 kb (E), and 5 kb (F) fragments.
Right(A-B): Cloning comparison using 3 kb fragments. Kit 10907 (A) yielded significantly more colonies than Supplier A* (B), demonstrating superior cloning efficiency.
Documents:
Safety Data Sheet
Manuals
10907_Manual_Ver.EN20230918.pdf
Pago y seguridad
Su información de pago se procesa de forma segura. No almacenamos detalles de la tarjeta de crédito ni tenemos acceso a la información de su tarjeta de crédito.
Consulta
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Preguntas frecuentes
El producto es solo para fines de investigación y no está destinado a uso terapéutico o diagnóstico en humanos o animales. Los productos y el contenido están protegidos por patentes, marcas comerciales y derechos de autor propiedad de Yeasen Biotechnology. Los símbolos de marca comercial indican el país de origen, no necesariamente el registro en todas las regiones.
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