Descripción
The HieffTM Plasmid Mini Kit adopts HieffTM DNA Column P3 and a new solution system, and uses the conventional alkali cracking method to separate plasmid DNA. In the extraction process, toxic organic substances such as phenol and chloroform are not needed to be extracted, and time-consuming isopropanol or ethanol precipitation is not needed. The HieffTM DNA Column P3 in the kit can specifically adsorb plasmid DNA, and does not adsorb protein, polysaccharide and other non-nucleic acid substances, so it is efficient, rapid and convenient. The extracted plasmid DNA has high purity and can be directly used in subsequent experiments such as DNA digestion, transformation and sequencing.
Feature
- Wide Sample Range: Suitable for routine small-scale plasmid extraction; processes 2–6 mL of bacterial culture per prep.
- High DNA Purity: Free from protein and RNA contamination; DNA is ready for downstream applications such as digestion, transformation, and sequencing.
- High DNA Yield: Columns can bind up to 60 μg of plasmid DNA.
Components
|
Components No. |
Name |
19001ES08 |
19001ES50 |
19001ES70 |
|
|
5 T |
50 T |
200 T |
|||
|
Part I |
19001-A |
RNase A (10 mg/mL) |
15 µL |
125 µL |
500 µL |
|
Part II |
19001-B |
RS Buffer P3* |
1.5 mL |
12.5 mL |
50 mL |
|
19001-C |
AC Buffer P3 |
500 µL |
5 mL |
20 mL |
|
|
19001-D |
HieffTM DNA Column P3 |
5 |
50 |
200 |
|
|
19001-E |
2 mL Collection Tube P3 |
5 |
50 |
200 |
|
|
19001-F |
Lysis Buffer(LB Buffer P3) |
1.5 mL |
12.5 mL |
50 mL |
|
|
19001-G |
Binding Buffer(BD Buffer P3) |
1.8 mL |
17.5 mL |
70 mL |
|
|
19001-H |
Deproteinization Buffer(PL Buffer P3*) |
1.6 mL |
16 mL |
63 mL |
|
|
19001-I |
Wash Buffer* |
1.3 mL |
13 mL |
50 mL |
|
|
19001-J |
Elution Buffer |
1 mL |
10 mL |
20 mL |
|
Shipping and Storage
Part I components are transported in ice bags and stored at -25~-15℃;
Part II components are transported at room temperature and stored at room temperature.
The product is valid for 12 months.
Application
Plasmid Extraction
Figures
1. High Yields
Figure 1. Comparison of Plasmid DNA Yields from Equal Volumes of Bacterial Culture
Plasmid yield: 19001 > Supplier M > Supplier F.
Documents:
Safety Data Sheet
Manuals
Pago y seguridad
Su información de pago se procesa de forma segura. No almacenamos detalles de la tarjeta de crédito ni tenemos acceso a la información de su tarjeta de crédito.
Consulta
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Preguntas frecuentes
El producto es solo para fines de investigación y no está destinado a uso terapéutico o diagnóstico en humanos o animales. Los productos y el contenido están protegidos por patentes, marcas comerciales y derechos de autor propiedad de Yeasen Biotechnology. Los símbolos de marca comercial indican el país de origen, no necesariamente el registro en todas las regiones.
Algunas aplicaciones pueden requerir derechos de propiedad intelectual adicionales de terceros.
Yeasen se dedica a la ciencia ética y cree que nuestra investigación debe abordar cuestiones críticas al tiempo que garantiza la seguridad y los estándares éticos.