Beskrivelse
Product Propertie
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Alternative Names |
CD120b, Etanercept, p75TBPII, p75TNFR, TNF RII, TNF-R2, TNF-R75, TNFR80, TNFRSF1B, TNFR2, TBPII, TNFBR, TNFR1B |
|
Species |
Human |
|
Expression Sequence |
Leu23-Asp257 |
|
Accession |
P20333-1 |
|
Tag |
C-hFc |
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Expression System |
HEK293 |
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Molecular Weight |
The protein has a predicted MW of 51.89 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
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Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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Appearance |
Lyophilized Powder |
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Biological Activity |
【ELISA Data】 Immobilized Human TNF alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA . 【ELISA Data】 Immobilized Human TNFR2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFR2 Antibody, hFc Tag with the EC50 of 19ng/ml determined by ELISA. |
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Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
Betaling og sikkerhed
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Forespørgsel
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FAQ
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