Beskrivelse
Product Properties
|
Synonyms |
ETGF; TGF-alpha; TGF type 1; TGFA |
|
Species |
Human |
|
Source |
HEK293 |
|
Sequence |
Val40-Ala89 |
|
Accession |
P01135-1 |
|
Tag |
N-hFc |
|
Molecular Weight |
The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by Bis-Tris PAGE and HPLC |
|
Activity |
ELISA Data: Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA
SPR Data: Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
lyophilized powder |
Storage
Store at -25°C to -15°C. The product is valid for 1 year upon receipt.
After reconstitution, store at -85°C to -65°C and use within 3 months.
Reconstitution
Centrifuge before opening the vial. It is recommended to dissolve the lyophilized protein in distilled water. The reconstitution concentration should exceed 100 μg/ml.
Note
1.Please operate with lab coats and disposable gloves, for your safety.
2.This product is for research use only.
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FAQ
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