Beskrivelse
Product Properties
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Synonyms |
αVβ3; alphaV; beta3; Integrin alpha V β 3 |
|
Source |
Mouse Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Phe31-Val988(ITGAV) acidic tail & Glu26-Asp717(ITGB3) basic tail.[Accession | P43406(ITGAV)&O54890(ITGB3)] |
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Molecular Weight |
The protein has a predicted MW of 131.48 kDa(ITGAV)&101.58 kDa(ITGB3). Due to enzyme lysis and glycosylation, the protein migrates to 110-135 kDa and 50-60 kDa based on Tris-Bis PAGE result. |
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Endotoxin |
Less than 1EU per μg by the LAL method. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC. |
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Activity |
SPR Data: Mouse ITGAV&ITGB3, hFc Tag captured on CM5 Chip via Protein A can bind Human Vitronectin, His Tag with an affinity constant of 3.37 nM as determined in SPR assay . |
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Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
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