Flag-Tag (DYKDDDDK), Mouse mAb _ 30503ES
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Flag-Tag (DYKDDDDK), Mouse mAb _ 30503ES
Flag-Tag (DYKDDDDK), Mouse mAb _ 30503ES
Tag antigens are widely used in recombinant protein detection for immunoblotting, immunoprecipitation, and immunoaffinity techniques due to their small molecular weight and minimal impact on fusion protein activity. The Flag tag is one of the most commonly used tag systems in recombinant protein research. It utilizes a short hydrophilic octapeptide (DYKDDDDK) fused to the N-terminus or C-terminus of the target protein, and can be subsequently removed by enterokinase. Flag antibodies can be used to detect the expression and intracellular localization of Flag-tagged fusion proteins, as well as for purification and...

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Flag-Tag (DYKDDDDK), Mouse mAb _ 30503ES

YeasenSKU: 30503ES20
Size: 20 μL
Pris:
$50.00
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Tag antigens are widely used in recombinant protein detection for immunoblotting, immunoprecipitation, and immunoaffinity techniques due to their small molecular weight and minimal impact on fusion protein activity. The Flag tag is one of the most commonly used tag systems in recombinant protein research. It utilizes a short hydrophilic octapeptide (DYKDDDDK) fused to the N-terminus or C-terminus of the target protein, and can be subsequently removed by enterokinase. Flag antibodies can be used to detect the expression and intracellular localization of Flag-tagged fusion proteins, as well as for purification and qualitative or quantitative analysis.

Product Properties

Synonyms

Flag-Tag; DDDDK tag; DDDDK-tag

Clonality

Monoclonal Antibody

SwissProt ID

/

Gene ID

/

Host

Mouse

Isotype

IgG2b

Clone No.

3F5

Immunogen

Synthetic Flag tag peptide coupled to KLH

Reactivity

All

Application

IF, IP, ELISA, WB

Conjugation

Unconjugated

Feature

Recommended dilution: WB: 1/500–1/5000; IF: 1/200; IP: 1/50–1/200; ELISA: 1/500–1/5000

Figure

Case Study 1: Western Blot

Figure 1: Western blot analysis of Flag-tagged fusion protein using Flag-Tag Mouse mAb at a dilution of 1:5000.

Figure 1: Western blot analysis of Flag-tagged fusion protein using Flag-Tag Mouse mAb at a dilution of 1:5000.

Case Study 2: IP

Figure 2: IP analysis of Flag-tagged fusion protein using Flag-Tag Mouse mAb at dilutions of 1:200, 1:100, and 1:50.

Figure 2: IP analysis of Flag-tagged fusion protein using Flag-Tag Mouse mAb at dilutions of 1:200, 1:100, and 1:50.

Case Study 3: Immunofluorescence

Figure 3: Immunofluorescence analysis of Flag Tag in HeLa cells transfected with a Flag-tag protein using Flag-Tag antibody (green) and DAPI (blue).

Figure 3: Immunofluorescence analysis of Flag Tag in HeLa cells transfected with a Flag-tag protein using Flag-Tag antibody (green) and DAPI (blue).

Storage

Store at -25°C to -15°C, valid for 1 year after receipt. Avoid repeated freeze-thaw cycles.

Documents:

Safety Data Sheet

Storage

Store at -25°C to -15°C, valid for 1 year after receipt. Avoid repeated freeze-thaw cycles.

FAQ

Citations

Documents

Applications

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FAQ

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