Beskrivelse
The Hieff™ Pro Soil/Stool DNA Kit is designed to extract high-purity DNA from 250 to 500 mg of soil samples. Utilizing superparamagnetic bead purification technology, this method eliminates the need for toxic phenol-chloroform extraction, offering a simple and convenient workflow. The extracted DNA is ready for direct use in downstream applications such as PCR, qPCR, microarray analysis, and Sanger or Next-Generation Sequencing (NGS). When paired with automated magnetic bead extraction instruments, the kit enables high-throughput nucleic acid extraction.
Features
Broad Applicability: Compatible with 250–500 mg soil and fecal samples.
Deep Impurity Removal: Effectively eliminates complex inhibitors from challenging soil matrices.
High-Purity Nucleic Acids: Yields DNA ready for direct use in PCR, qPCR, and NGS/Third-Generation Sequencing.
High-Throughput Compatibility: Supports automated instruments for rapid, batch processing.
Components
|
Category |
Components No. |
Name |
18527ES48 (48 T) |
|
Part Ⅰ |
18527-A |
Proteinase K |
1.1 mL/tube × 1 |
|
Part II |
18527-B |
Magnetic Bead Suspension |
1.1 mL/tube × 1 |
|
18527-C |
Lysis Buffer |
30 mL/bottle × 1 |
|
|
18527-D |
Grinding Tube |
8 tubes/bag × 1 bag |
|
|
18527-E |
Lysis Enhancer SE |
3 mL/bottle × 1 |
|
|
18527-F |
Precipitation Solution |
7 mL/bottle × 1 |
|
|
18527-G |
Lysis Buffer LB |
30 mL/bottle × 1 |
|
|
18527-H |
Deproteinization Solution PL* |
40 mL/bottle × 1 |
|
|
18527-I |
Wash Buffer AW* |
40 mL/bottle × 1 |
|
|
18527-J |
Wash Buffer BW* |
40 mL/bottle × 1 |
|
|
18527-K |
Elution Buffer DE |
6 mL/bottle × 1 |
[Note]: Add 6.3 mL isopropanol into the bottle of 18527-H Deproteinization Solution PL.
Add 12 mL isopropanol into the bottle of 18527-I Wash Buffer AW.
Add 34 mL absolute ethanol into the bottle of 18527-J Wash Buffer BW.
Storage
Store Part I at 2–8°C, valid for 18 months.
Store Part II at room temperature, valid for 18 months.
Figure
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Case Study 1: High-Humic-Acid Black Soil Samples
Figure 1. Agarose gel electrophoresis analysis of DNA extracted from high-humic-acid black soil samples.
Compared with PBS treatment, the proprietary precipitation solution:
- Increased DNA yield by approximately 3-fold
- Significantly reduced humic acid contamination
- Improved A260/230 and A260/280 purity ratios
- Produced cleaner DNA suitable for downstream amplification
Case Study 2: High-Humic-Acid Sludge Samples
Figure 2. Comparison of DNA extraction performance from high-humic-acid sludge samples.
The 18527ES kit demonstrated:
- Higher DNA recovery
- Strong and intact electrophoretic bands
- Minimal DNA degradation
- Superior removal of inhibitory substances
Agarose gel analysis showed bright, well-defined genomic DNA bands with minimal smearing, indicating excellent DNA integrity and extraction efficiency.
Case Study 3: Human Fecal Samples
Human fecal samples (200 mg input) were processed using the Yeasen Enhanced Soil DNA Extraction Kit (18527ES) on the Yeasen AP-48 Automated Nucleic Acid Extraction System and compared with a leading supplier's column-based manual extraction kit (Supplier T). All samples were eluted in 80 μL.
Figure 3. DNA yield and purity comparison of human fecal samples extracted using 18527ES and Supplier T.
Case Study 4: Water Filter Membrane Samples
Figure 4. DNA extraction performance comparison for water filter membrane samples.
Water filter membrane samples were extracted using the 18527ES kit and compared with Supplier M and Supplier J using the same automated extraction platform and elution conditions.
Results
The 18527ES kit achieved DNA yields comparable to both competing products, demonstrating reliable recovery of microbial DNA from low-biomass environmental water samples.
Case Study 5: Biogas Residue and Straw Compost Samples
Biogas digestate and straw compost samples were processed using the 18527ES kit alongside Supplier M and Supplier J under identical extraction conditions.
Results
The 18527ES kit produced DNA yields comparable to Supplier J and consistently higher than Supplier M, highlighting its strong capability for extracting DNA from organic matter-rich agricultural samples.
Figure 5. DNA extraction performance comparison for biogas residue and straw compost samples.
Documents:
Safety Data Sheet
Manuals
Related Blogs:
Humic Acid Removal Breakthrough: Enhanced Soil DNA Extraction Kit for Complex Environmental Samples
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FAQ
Produktet er kun til forskningsformål og er ikke beregnet til terapeutisk eller diagnostisk brug hos mennesker eller dyr. Produkter og indhold er beskyttet af patenter, varemærker og ophavsrettigheder ejet af Yeasen Biotechnology. Varemærkesymboler angiver oprindelseslandet, ikke nødvendigvis registrering i alle regioner.
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