Beskrivelse
The SuperMethyl™ Max Bisulfite Conversion Kit from Ellis Bio offers the exceptionally mild yet powerful bisulfite conversion for DNA methylation analysis. Featuring a newly engineered ultra-mild bisulfite conversion formulation and a magnetic bead-based DNA purification method, this kit simplifies the methyl conversion workflow and achieves efficient conversion with minimal DNA degradation. Complete bisulfite conversion and DNA purification can be accomplished in 3 hours or less, preserving DNA integrity even with ultra-low input samples, while consistently achieving 99.8% conversion efficiency. Its broad applicability makes it a valuable tool for both research and translational settings.
Designed for compatibility and consistent performance in sensitive and/or low-input DNA samples, the SuperMethyl™ Max Bisulfite Conversion Kit delivers reliable, reproducible results, making it the preferred choice for precision-focused researchers.
Features
- Ultra-Mild Conversion Chemistry:Delivers excellent DNA library yield and larger library insert sizes, preserving sample integrity.
- Low-Input Friendly:Efficiently processes as little as 100 pg of DNA, ideal for limited or precious samples.
- Exceptional Conversion Efficiency:Achieves 99.8% C-to-T conversion, ensuring unmatched accuracy in methylation analysis.
- Uniform GC Coverage:Provides more even GC representation, enhancing coverage across promoters and GC-rich regions.
- Simplified, Rapid Workflow:Up to 3× faster with 5× less hands-on time than enzyme-based methods.
- Convenient Bead-Based Purification Format:Streamlined purification for improved yield and ease of use. Automation liquid handler friendly.
Component
|
Component |
Volume/Ǫuantity |
Storage |
|
Kit Capacity |
24 Tests |
Please see storage recommendations for individual components |
|
Conversion Reagent ◉ |
2 mL |
4 °C (recommended) |
|
Preparation Reagent ◉ |
120 µL |
Room temperature |
|
Enhancer A ◉ |
120 µL |
Room temperature |
|
Enhancer B ◉ |
240 µL |
4 °C (recommended) |
|
Binding Buffer |
22 mL |
Room temperature |
|
Wash Buffer (concentrate)* |
7 mL* |
Room temperature |
|
Desulphonation Buffer |
6 mL |
Room temperature |
|
Elution Buffer |
1 mL |
Room temperature |
|
Purification Beads ◉ |
400 µL |
Room temperature |
|
* Wash Buffer requires the addition of 28 mL 100% ethanol (EtOH) before first use. |
||
Shipping Conditions
No Cold Chain Required; Shipped at Room Temperature
Precautions / Handling Instructions
Upon receipt of the kit, store the Conversion Reagent and Enhancer B at 4°C.
Figures
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