Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES
Rul over billedet for at zoome ind Klik på billedet for at zoome
Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES
Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES
Bsu DNA polymerase (Large Fragment) is derived from Bacillus subtilis. This enzyme retains the 5′-to-3′ polymerase activity of Bsu DNA polymerase I but lacks the 5′-to-3′ exonuclease domain. The large fragment itself lacks 3′-to-5′ exonuclease activity. It is a DNA isothermal amplification polymerase with strand-displacement activity and is mainly used in Recombinase Polymerase Amplification (RPA). Product Information Catalog No. 14415ES72 / 14415ES80 / 14415ES90 / 14415ES97 Size 2,500 U / 5,000 U / 50,000 U / 250,000 U Unit Definition One unit (U) is defined as the amount of enzyme...

Frequently Bought Together

Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES

YeasenSKU: 14415ES72
Size: 2,500 U
Pris:
$715.00
Lager:
På lager

Bsu DNA polymerase (Large Fragment) is derived from Bacillus subtilis. This enzyme retains the 5′-to-3′ polymerase activity of Bsu DNA polymerase I but lacks the 5′-to-3′ exonuclease domain. The large fragment itself lacks 3′-to-5′ exonuclease activity. It is a DNA isothermal amplification polymerase with strand-displacement activity and is mainly used in Recombinase Polymerase Amplification (RPA).

Product Information

Catalog No.

14415ES72 / 14415ES80 / 14415ES90 / 14415ES97

Size

2,500 U / 5,000 U / 50,000 U / 250,000 U

Unit Definition

One unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material within 30 min at 37°C.

Storage Buffer

25 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C.

Heat Inactivation

75°C for 20 min.

Component Information

Product Name

14415ES72
(2,500 U)

14415ES80
(5,000 U)

14415ES90
(50,000 U)

14415ES97
(250,000 U)

Bsu DNA Polymerase (Large Fragment, 50 U/μL)

50 μL

100 μL

1 mL

5 × 1 mL

Feature

  • Due to the lack of 3′-to-5′ exonuclease activity, Bsu DNA polymerase (Large Fragment) cannot remove unpaired 3′ overhangs and is therefore not suitable for generating blunt ends.
  • At 25°C, Bsu DNA polymerase (Large Fragment) retains 50% activity, which is twice that of Klenow Fragment (3′-to-5′ exo-) at the same temperature.
  • Storage buffer: 25 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C.
  • Heat inactivation: 75°C for 20 min.

Storage

Store at -25°C to -15°C. The shelf life is 2 years.

Documents:

Safety Data Sheet

14415_MSDS_HB260819

Manuals:

14415_Manual_HB20260818

Storage

Store at -25°C to -15°C. The shelf life is 2 years.

FAQ

Citations

Documents

Applications

Du kan også lide

Forespørgsel

FAQ

Produktet er kun til forskningsformål og er ikke beregnet til terapeutisk eller diagnostisk brug hos mennesker eller dyr. Produkter og indhold er beskyttet af patenter, varemærker og ophavsrettigheder ejet af Yeasen Biotechnology. Varemærkesymboler angiver oprindelseslandet, ikke nødvendigvis registrering i alle regioner.

Visse applikationer kan kræve yderligere tredjeparts intellektuelle ejendomsrettigheder.

Yeasen er dedikeret til etisk videnskab og mener, at vores forskning bør adressere kritiske spørgsmål og samtidig sikre sikkerhed og etiske standarder.