Beskrivelse
Hieff Unicon™ Hotstart J-Taq DNA Polymerase, Glycerol-free (40 U/μL) is a hot-start DNA polymerase. This product utilizes antibody-mediated inhibition, providing high amplification sensitivity and specificity. The inhibitory antibody is completely inactivated and the DNA polymerase activity is fully released after a 30-second heat activation step at the initial denaturation temperature. Using this hot-start Taq enzyme effectively suppresses non-specific amplification caused by primer mis-annealing. This glycerol-free formulation is specifically designed for lyophilized reagent preparation.
Features
Rapid Activation: Taq polymerase achieves full activity after a quick 30 s incubation at 95 °C, ensuring immediate and efficient amplification.
High Sensitivity & Specificity: Provides strong amplification performance even with low template input, minimizing non-specific products.
Broad Instrument Compatibility: Works seamlessly with a wide range of standard PCR and real-time PCR platforms.
Lyophilization-Ready: Formulation supports freeze-drying, enabling long-term storage, reduced shipping costs, and simplified reagent handling.
Components
|
Name |
14326ES02 |
14326ES20 |
14326ES30 |
|
Hotstart J-Taq DNA Polymerase, Glycerol-free ( 40 U/μL) |
50 μL |
500 μL |
2×1 mL |
Storage
This product should be stored at -25~-15℃ for 1 year.
Application
Gene Expression Analysis; Low-Copy Target Amplification; Lyophilized PCR/qPCR.
Figures
1. Consistent Performance & Strong Stability
Figure 1. Stability of Yeasen glycerol-free enzyme (14326ES).
The glycerol-free enzyme showed the same amplification performance as the glycerol-containing enzyme across COVID-19 pseudovirus templates (105–103 copies/mL). After 54 days at 37 °C, its performance remained unchanged, confirming excellent thermal stability.
Documents:
Safety Data Sheet
Manuals
Betaling og sikkerhed
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Forespørgsel
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FAQ
Produktet er kun til forskningsformål og er ikke beregnet til terapeutisk eller diagnostisk brug hos mennesker eller dyr. Produkter og indhold er beskyttet af patenter, varemærker og ophavsrettigheder ejet af Yeasen Biotechnology. Varemærkesymboler angiver oprindelseslandet, ikke nødvendigvis registrering i alle regioner.
Visse applikationer kan kræve yderligere tredjeparts intellektuelle ejendomsrettigheder.
Yeasen er dedikeret til etisk videnskab og mener, at vores forskning bør adressere kritiske spørgsmål og samtidig sikre sikkerhed og etiske standarder.