YeaRed is a unique, lipophilic macromolecular nucleic acid dye that cannot penetrate cell membranes and therefore does not enter living cells. It exhibits negligible volatility and sublimation, minimizing the risk of inhalation exposure. Ames testing has confirmed that YeaRed is completely non-mutagenic at standard gel staining concentrations, making it a safe and non-toxic alternative to ethidium bromide (EB).

YeaRed shares identical spectral properties with EB and can be visualized using standard UV transilluminators equipped with 300 nm excitation without requiring any changes to filters or imaging equipment. It is compatible with both agarose and polyacrylamide gel electrophoresis for staining double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and RNA. Users may choose either pre-cast staining (gel casting method) or post-electrophoresis staining (soak staining method), offering flexibility and convenience.

Features

• High Sensitivity: Comparable to EB sensitivity
• Strong Stability: Microwaveable, stored at room temperature
• Wide applicability: Applicable for DNA or RNA staining
• Safe and Non-Toxic: Ames test shows no caccinogenic toxicity
• Easy to Dispose: Waste Yeared can be directly disposed of without environmental pollution
• Perfect Replacement: With the same spectral properties as EB

Components

Storage

This product should be stored at -25℃~-15℃ for up to 2 years.

Figure

Figure. Comparable electrophoresis performance between YeaRed and EB.

Samples: 100 bp DNA Ladder and 2000 bp DNA Marker, with loading volumes of 2, 3, 4, and 5 μL. Electrophoresis was performed at 140 V for 40 min.

Documents:

Safety Data Sheet

10203_MSDS_HB251111_EN.PDF

Manuals

10203_Manual_Ver.EN20251111.pdf

Publications Using This Product

[1]. Liu C, Zou G, et al. 5-Formyluracil as a Multifunctional Building Block in Biosensor Designs[J]. Angew Chem Int Ed Engl. 2018 Jul 26;57(31):9689-9693. (IF 11.992)  

[2]. Yang X, Gao F, Zhang W, et al. “Star” miR-34a and CXCR4 antagonist based nanoplex for binary cooperative migration treatment against metastatic breast cancer[J]. Journal of Controlled Release, 2020, 326: 615-627. (IF7.727)  

[3]. Lin Q, Ye X, Yang B, et al. Real-time fluorescence loop-mediated isothermal amplification assay for rapid and sensitive detection of Streptococcus gallolyticus subsp. gallolyticus associated with colorectal cancer[J]. Analytical and bioanalytical chemistry, 2019, 411(26): 6877-6887. (IF6.35)  

[4]. Lin Q, Ye X, Huang Z, et al. Graphene Oxide-Based Suppression of Nonspecificity in Loop-Mediated Isothermal Amplification Enabling Sensitive Detection of Cyclooxygenase-2 mRNA in Colorectal Cancer[J]. Analytical chemistry, 2019. (IF6.35)  

[5]. Wang W, Nie A, Lu Z, et al. Catalytic hairpin assembly-assisted lateral flow assay for visual determination of microRNA-21 using gold nanoparticles[J]. Microchimica Acta, 2019, 186(9): 661. (IF5.479) 

[6]. Wang H, Chen Y, Zhang J, et al. Using Nrf2/antioxidant response element-dependent signaling to assess the toxicity potential of fly ash particles[J]. Ecotoxicology and environmental safety, 2019, 170: 172-179. (IF4.527)  

[7]. Wang, J., et al., Delaminated Layered Double Hydroxide Nanosheets as an Efficient Vector for DNA Delivery[J]. J Biomed Nanotechnol, 2016. 12(5): p. 922-33. (IF 4.521)  

[8]. Cheng H, Fan X, et al. Cyclodextrin-Based Star-Like Amphiphilic Cationic Polymer as a Potential Pharmaceutical Carrier in Macrophages[J]. Macromol Rapid Commun. 2018 May 28:e1800207. (IF 4.441) 

[9]. Wei, X., et al., GDNF-expressing STO feeder layer supports the long-term propagation of undifferentiated mouse spermatogonia with stem cell properties[J]. Sci Rep, 2016. 6: p. 36779. (IF 4.259)

[10]. Liu, N., et al., Development of a multiplex loop-mediated isothermal amplification method for the simultaneous detection of Salmonella spp. and Vibrio parahaemolyticus[J]. Sci Rep, 2017. 7: p. 45601. (IF 4.259)