Booster DNA/RNA Transfection Reagent is an advanced nucleic acid transfection reagent developed based on novel polymeric materials. Its unique molecular design significantly reduces cytotoxicity and features a smart anti-enzymatic degradation mechanism that protects nucleic acids from intracellular enzymatic degradation during delivery, thereby maximizing transfection efficiency. This reagent is suitable for various types of nucleic acids including DNA, siRNA, miRNA, mRNA, and ASO, and has been validated for efficient transfection in cell lines such as 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW267.4, HCT116, as well as primary cells, providing reliable tools for gene research.

Features

Low Cytotoxicity: The novel polymer replaces traditional liposomes to reduce cytotoxicity and improve cell viability.

Fast Release and Higher Efficiency: Fast release minimizes intracellular nucleic acid degradation, achieving >90% transfection efficiency (measured by flow cytometry).

Premium Options for Difficult-to-transfect cell lines: Sensitive and primary cells.

Proven Performance, Broad Cell Line Coverage: Achieves high efficiency in 200+ various cell lines including 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, and HCT116 and primary cells, with consistent and reproducible results.

One Reagent, All Nucleic Acids: Delivers DNA, siRNA, miRNA, mRNA, and ASO with high efficiency—even up to 15 kb fragments.

Components

Shipping and Storage

This product should be stored at 2~8℃ for 12 months.

Application

Cell Transfection; DNA Transfection; mRNA/SiRNA Transfection; 

Figures

1) High-Efficiency Primary Cell BMDM Transfection

Figure 1. Plasmid DNA transfection in primary bone marrow-derived macrophages (BMDMs) using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.

2) High-Efficiency DNA Transfection

 Figure 2. DNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior DNA transfection efficiency of Booster.

3) High-Efficiency mRNA Transfection

 Figure 3. mRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior mRNA transfection efficiency of Booster.

4) High-Efficiency siRNA Transfection

 Figure 4. siRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior siRNA transfection efficiency of Booster.

Customer Feedback

1) High-Efficiency Primary Dermal Fibroblasts Transfection

 Figure 5. Plasmid EGFR DNA transfection in primary dermal fibroblasts using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.

2) High-Efficiency Porcine Alveolar Macrophages Transfection

Figure 6. DNA transfection in primary Porcine Alveolar Macrophages (PAMs) using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.

Documents:

Safety Data Sheet

40801_MSDS_HB250715_EN.PDF

Manuals

40801_Manual_Ver.EN20250807.pdf

Related Blog:

Protocol for Transfection of mRNA into Cells

Protocol for Transfection of siRNA into Cells

Beyond Liposomes: The Next Generation Booster DNA/RNA Transfection Reagent

Protocol for Transfection of Primary Bone Marrow-Derived Macrophages (BMDMs)

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