Tanım
IL-33 is a 30 kDa pro-inflammatory protein. IL-33 is constitutively expressed in smooth muscle and airway epithelium. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following stimulation with IL-1 alpha or IL-1 beta. The C-terminal fragment of mature IL-33 binds to and triggers signaling through the mast cell IL-1 R4/ST2L, an orphan receptor long implicated in enhancing Th2 cell responses. Subsequently, IL-33 and ST2L associate with IL-1R AcP to form a ternary signaling complex. Stimulation of Th2-polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion. Administration of mature IL-33 in vivo promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues. Full-length and mature human IL-33 share 52-58% amino acid sequence identity with mouse and rat IL-33.
This product is provided as a lyophilized powder and features high purity, high activity, and low endotoxin.
Product Information
|
Product Information. |
Catalog Number |
Size |
|
Recombinant Human PDGF-AB Protein |
90369ES05 |
2 μg |
|
90369ES10 |
10 μg |
|
|
90369ES50 |
50 μg |
|
|
90369ES60 |
100 μg |
|
|
90369ES80 |
1 mg |
Performance Parameters
|
Synonyms |
IL33, IL-33, interleukin 33, Interleukin-1 family member 11, interleukin -33 |
|
Source |
E.coli |
|
Accession |
O95760 |
|
Molecular Weight |
Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing159 amino acids. |
|
AA Sequence |
Ser112-Thr270 |
|
Tag |
No Tag |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Purity |
> 97% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 10^7 IU/mg. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PBS, 150 mM NaCl, 1 mM EDTA, pH7.4. |
|
Appearance |
Lyophilized Powder |
Storage
Store at -25 ~ -15°C. Valid for 1 year upon receipt.
After reconstitution, store at 2 ~ 8°C for up to 30 days, or at -85 ~ -65°C for up to 3 months.
Reconstitution Method
Centrifuge before opening the vial to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, and can be further diluted according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer. Aliquot into single-use portions, freeze at -80°C, and avoid repeated freeze-thaw cycles.
Notes
1. For your safety and health, please wear a lab coat and disposable gloves during operation.
2. This product is for research use only.
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