Tanım
Interleukin-18 (IL-18) is a pro-inflammatory cytokine belonging to the IL-1 superfamily, produced by macrophages and other cell types. Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18. In the presence of IL-12 or IL-15, it induces Th1 cells, natural killer (NK) cells, and CD4+ T cells to produce interferon-gamma (IFN-γ), thereby amplifying Type I cellular immunity. IL-18 serves as a key cytokine in the immune response against intracellular bacteria and viruses. Furthermore, IL-18 enhances the proliferation, survival, and cytotoxic activity of NK cells and macrophages by upregulating the expression of perforin, granzymes, and FasL, thereby strengthening the clearance of tumor and infected cells.
Features
High Purity: >95% purity to avoid immune responses caused by residual Host Cell Proteins (HCP) or DNA.
Ultra-Low Endotoxin: <10 EU/mg to minimize the risk of fever or inflammation.
Batch-to-Batch Consistency: Ensures stability and reproducibility in cell culture applications.
Regulatory Compliance: Meets regulatory requirements for cell therapy products and supports Investigational New Drug (IND) applications.
Animal-Origin Free (AOF): Free from animal viruses, pathogens, and exogenous factor contamination, ensuring maximum safety.
Components
|
Name |
90276ES10 |
90276ES50 |
90276ES80 |
|
Recombinant Human IL-18 GMP Protein |
10 μg |
50 μg |
1 mg |
Performance parameters
|
Synonyms |
Interleukin 18,IGIF, IL-1F4, IL-1g, IL18 |
|
Source |
Human IL-18 Protein is expressed from E.coli without tag. |
|
Sequence |
Tyr 37 - Asp 193，N-terminus: Met |
|
Molecular Weight |
Approximately 17.5 kd |
|
Accession |
Q14116 |
|
Biological Activity |
Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 3-36 ng/mL. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
＜0.01 EU/μg protein as determined by LAL method. |
|
Sterility |
Negative. |
|
Mycoplasma |
Negative. |
|
Vitro Virus |
Negative. |
|
Host Cell Protein |
Less than 0.5 ng/μg when tested by ELISA. |
|
Host Cell DNA |
Less than 20 ng/mg when tested by qPCR. |
|
Formulation |
0.22 μm filtered concentrated solution in PBS. |
|
Physical Appearance |
Lyophilized Powder |
Storage
Store at -25°C to -15°C. Shelf life is 3 years. After reconstitution, store at -85°C to -65°C for up to 3 months.
Application
T Cell Expansion: Promotes T cell proliferation, prevents functional exhaustion, and enhances transduction efficiency in TCR-T therapies.
Dendritic Cell Generation: Acts as a key driver for the maturation of dendritic cells from monocytes.
Immune Cell Culture: Enhances the anti-tumor cytotoxic activity of CIK, NK, and macrophage cells.
Figure
1. High purity
Figure 1. Human IL-18 GMP Protein on SDS-PAGE under reduced condition. The purity is greater than 95%.
2. SEC-HPLC
Figure 2. The purity of Human IL-18 is greater than 95% as determined by SEC-HPLC
3. Cell Based Assay
Figure 3. Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 3-36 ng/mL.
Documents:
Safety Data Sheet
Manuals
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