Frequently Bought Together
The Cell Species Identification Kit (Multiplex PCR Method) is designed for the identification of cell substrate origins, ensuring correct cell line identity and absence of cross-contamination from other cell lines. It enables quality control of raw materials in cell-based biopharmaceuticals.
This kit is based on multiplex PCR amplification of mitochondrial genes from animal cells, including cytochrome b, cytochrome c oxidase I (COX I), and cytochrome c oxidase II (COX II). Species origin is determined by analyzing the amplification products via agarose gel electrophoresis.
Features
Broad Species Compatibility – Accurately detects 9 different cell species, including human (HEK293), mouse (SP2/0), bovine (MDBK), porcine (PK15), canine (MDCK), Chinese hamster (CHO), African green monkey (Vero), feline (CRFK), rat (C6) and rhesus monkey (LLC-MK2).
High Specificity – Reliably identifies contaminating cells even in the presence of primary cells (HEK293, MDCK, Vero, CHO).
Reliable Results – Based on amplicon band number and size for clear interpretation.
High Quality & Stable Supply – Ensures consistent performance and reliable availability.
Specifications
|
Cat NO. |
41341ES25 / 41341ES50 |
|
Size |
25 T / 50 T |
Components
|
category |
Components No. |
Name |
41341ES25 |
41341ES50 |
|
Part I |
41341-C |
Cell Extraction Buffer |
2 × 1.25 mL |
4 × 1.25 mL |
|
Part II |
41341-A |
2× Multiplex PCR Buffer |
312.5 μL |
625 μL |
|
41341-B |
Primers Mix |
112.5 μL |
225 μL |
|
|
41341-D |
Proteinase K (20 mg/mL) |
50 μL |
100 μL |
|
|
41341-E |
DL500 DNA Marker |
125 μL |
250 μL |
|
|
41341-F |
DEPC-Treated Water |
1.25 mL |
2 × 1.25 mL |
|
|
41341-G |
Positive Control |
25 μL |
50 μL |
Application
Cell species identification
Figures
1. Broad Species Compatibility
Figure 1. Broad species compatibility. Clear amplification results were obtained across different species, including human (HEK293), mouse (SP2/0), bovine (MDBK), porcine (PK15), canine (MDCK), Chinese hamster (CHO), African green monkey (Vero), feline (CRFK), rat (C6), and rhesus monkey (LLC-MK2).
Storage
FAQ
Citations
Applications
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