Enterokinase Activity Assay Kit _ 20393ES

YeasenSKU: 20393ES60

Size: 100 T
Fiyat:
Satış fiyatı$1,000.00
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Stokta var

Enterokinase (also known as enteropeptidase, EC 3.4.21.9) is a key enzyme that initiates protein digestion in the intestine. It precisely activates trypsinogen to trypsin, which then cascade-activates chymotrypsinogen and procarboxypeptidase. Its expression is specifically localized in differentiated intestinal cells and goblet cells, making it both a core regulator of nutritional digestion and a reliable marker of intestinal epithelial maturation.
The Enterokinase Activity Assay Kit is a rapid method for determining enterokinase activity in biological samples. This method detects enterokinase activity by cleaving a 7-amino-4-trifluoromethylcoumarin (AFC)-labeled peptide substrate containing an enterokinase recognition sequence. The intensity of the fluorescent product AFC (excitation wavelength Ex=380 nm, emission wavelength Em=500 nm) generated by the reaction is proportional to the enzyme activity in the sample.

Product Information

Component No.

Component Name

20393ES60 (100 T)

20393-A

200× Enterokinase Substrate

24 μL

20393-B

1 mM AFC Standard

100 μL

20393-C

Enterokinase Assay Buffer

60 mL

Properties

Cat#

Component Name

20393ES60 (100T)

20393-A

200x Enterokinase Substrate

24 uL

20393-B

1 mM AFC Standard

100 uL

20393-C

Enterokinase Assay Buffer

60 mL

Figures

Figure 1. AFC standard curve.

Figure 1. AFC standard curve.

Figure 2. Sensitivity of the Yeasen Enterokinase (Cat#20395ES) detection kit. Data were collected at 25°C for 60 min according to the kit instructions. Figure 2. Sensitivity of the Yeasen Enterokinase (Cat#20395ES) detection kit. Data were collected at 25°C for 60 min according to the kit instructions.

Storage

Store at -25 to -15℃. The unopened kit is valid for 1 year; after opening, valid for 6 months.

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