Tanım
This Kit is designed for rapid and efficient RNA extraction from Gram-positive and Gram-negative bacteria.Utilizing a proprietary silica- membrane column technology combined with optimized buffers, this kit effectively removes contaminants such as proteins, metabolites, and genomic DNA. The purified RNA is suitable for downstream applications including RT-PCR, RT-qPCR, in vitro transcription, and molecular cloning.
Specifications
|
Cat.No. |
19301ES50 |
|
Size |
50 T |
Components
|
Category |
Components No. |
Name |
19301ES50 |
|
Part I |
19301-A |
Lysozyme |
20 mg |
|
Part II |
19301-B |
DNA-Removing Column B4 |
50 units |
|
19301-C |
Hieff™ RNA Column B4 |
50 units |
|
|
19301-D |
2 mL Collection Tube B4 |
100 units |
|
|
19301-E |
Lysis Buffer(LB Buffer B4) |
25 mL |
|
|
19301-F |
PL Buffer B4 |
40 mL |
|
|
19301-G |
Wash Buffer B4* |
13 mL(add 52 mL ethanol before use) |
|
|
19301-H |
TE Buffer pH 8.0 |
6 mL |
|
|
19301-I |
RNase-free H2O |
5 mL |
Features
Ø Wide applicability: can be used for bacterial cultures such as Escherichia coli, Pseudomonas aeruginosa, and Gram-positive bacteria.
Ø Quick and easy: the operation can be completed within 40 minutes.
Ø High RNA purity: DNase I on-column digestion effectively removes gDNA.
Ø Safe and environmentally friendly: no need to use organic matter such as phenol and chloroform for extraction.
Applications
Bacterial RNA extraction；RNA extraction from Gram-positive bacteria；Gram-negative bacteria, etc.
Shipping and Storage
Part I components should be stored at 4~8℃ for one year.
Part II components should be stored at room temperature for one year.
Figures
|
Suppliers |
Concentration |
OD260/OD280 |
OD260/OD230 |
|
Yeasen-19031ES |
159.40/193.18 |
1.895/1.925 |
2.177/2.101 |
|
Supplier T* |
182.01/179.31 |
1.855/1.922 |
0.728/1.206 |
Table 1. E. coli extract product concentration
Figure 1. Electrophoresis of E. coli RNA extraction products
Take 4 portions of 2 mL of E. coli suspension, use 19301 and similar products from supplier T brand to extract total RNA, and elute with 50 ul RNase-free H2O. Then take 1 ul of the eluate to determine the concentration. Take 5 ul of the eluate to identify the RNA integrity and band brightness. The results show that the 19301 extraction product has a high yield and good RNA integrity.
Documents:
Safety Data Sheet
19301_Manual_Ver. EN20250514_EN.pdf
Citations & References:
[1] Ma X, Hou M, Liu C, Li J, Ba Q, Wang H. Cadmium accelerates bacterial oleic acid production to promote fat accumulation in Caenorhabditis elegans. J Hazard Mater. 2022;421:126723. doi:10.1016/j.jhazmat.2021.126723(IF:10.588)
[2] Wang Y, Hu W, Deng Z, He X. Rapid identification of magnesium ascorbyl phosphate utilizing phosphatase through a chromogenic change-coupled activity assay. Appl Microbiol Biotechnol. 2021;105(7):2901-2909. doi:10.1007/s00253-021-11229-7(IF:4.813)
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Sorgu
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SSS
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